Predicting thresholds for population replacement gene drives.

Autor: Janzen A; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, MN, USA.; Biotechnology Institute, University of Minnesota, Saint Paul, 55108, MN, USA., Pothula R; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, MN, USA.; Biotechnology Institute, University of Minnesota, Saint Paul, 55108, MN, USA., Sychla A; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, MN, USA.; Biotechnology Institute, University of Minnesota, Saint Paul, 55108, MN, USA., Feltman NR; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, MN, USA.; Biotechnology Institute, University of Minnesota, Saint Paul, 55108, MN, USA., Smanski MJ; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, MN, USA. smanski@umn.edu.; Biotechnology Institute, University of Minnesota, Saint Paul, 55108, MN, USA. smanski@umn.edu.
Jazyk: angličtina
Zdroj: BMC biology [BMC Biol] 2024 Feb 19; Vol. 22 (1), pp. 40. Date of Electronic Publication: 2024 Feb 19.
DOI: 10.1186/s12915-024-01823-2
Abstrakt: Background: Threshold-dependent gene drives (TDGDs) could be used to spread desirable traits through a population, and are likely to be less invasive and easier to control than threshold-independent gene drives. Engineered Genetic Incompatibility (EGI) is an extreme underdominance system previously demonstrated in Drosophila melanogaster that can function as a TDGD when EGI agents of both sexes are released into a wild-type population.
Results: Here we use a single generation fitness assay to compare the fecundity, mating preferences, and temperature-dependent relative fitness to wild-type of two distinct genotypes of EGI agents. We find significant differences in the behavior/performance of these EGI agents that would not be predicted a priori based on their genetic design. We report a surprising temperature-dependent change in the predicted threshold for population replacement in an EGI agent that drives ectopic expression of the developmental morphogen pyramus.
Conclusions: The single-generation fitness assay presented here could reduce the amount of time required to estimate the threshold for TDGD strategies for which hybrid genotypes are inviable. Additionally, this work underscores the importance of empirical characterization of multiple engineered lines, as behavioral differences can arise in unique genotypes for unknown reasons.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje