In planta expression of specific single chain fragment antibody (scFv) against nucleocapsid protein of fig mosaic virus (FMV).

Autor: Jahandideh M; Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran., Rakhshandehroo F; Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address: Rakhshandehroo_fa@srbiau.ac.ir., Safarnejad MR; Department of Plant Viruses, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran., Sahraroo A; Department of Horticultural sciences, Faculty of Agricultural Science, Guilan University, Rasht, Iran., Elbeaino T; Istituto Agronomico Mediterraneo di Bari (CIHEAM-IAMB), Via Ceglie 9, Valenzano, Bari 70010, Italy.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 May; Vol. 326, pp. 114904. Date of Electronic Publication: 2024 Feb 17.
DOI: 10.1016/j.jviromet.2024.114904
Abstrakt: Fig mosaic virus (FMV) is recognized as the main viral agent associated with the mosaic disease (MD) of fig trees (Ficus carica). Due to its worldwide occurrence, FMV represents the most significant global threat to the production of fig fruit. A disease management strategy against the MD in fig orchards has never been effective; and therefore, expression of recombinant antibody in plant cells could provide an alternative approach to suppress FMV infections. In this study we focused on expressing a specific recombinant antibody, a single-chain variable fragment (scFv), targeting the nucleocapsid protein (NP) of FMV in planta. To accomplish this objective, we inserted the scFv gene into a plant expression vector and conducted transient expression in leaves of Nicotiana tabacum cv. Samson plants. The construct was transiently expressed in tobacco plants by agroinfiltration, and antibody of the anticipated size was detected by immunoblotting. The produced plantibody was then assessed for specificity using ELISA and confirmed by Western blot analysis. In this study, the plantibody developed against FMV could be considered as a potential countermeasure to the infection by conferring resistance to MD.
Competing Interests: Declaration of Competing Interest The authors (Mahsa Jahandideh; Farshad Rakhshandehroo; Mohammadreza Safarnejad; Amir Sahraroo and Toufic Elbeaino) declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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