Ehrlich ascites carcinoma provokes renal toxicity and DNA injury in mice: Therapeutic impact of chitosan and maitake nanoparticles.

Autor: Abosharaf HA; Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt., Gebreel DT; Medical Equipment Department, Faculty of Allied Medical Sciences, Pharos University, Alexandria, Egypt., Allam S; Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt., El-Atrash A; Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt., Tousson E; Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Jazyk: angličtina
Zdroj: Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2024 Apr; Vol. 134 (4), pp. 472-484. Date of Electronic Publication: 2024 Feb 18.
DOI: 10.1111/bcpt.13988
Abstrakt: In this study, the impact of chitosan (CS) and maitake (GF) nanoparticles towards the renal toxicity induced by Ehrlich ascites carcinoma (EAC) in vivo model was conducted. Besides benchmark negative control group, EAC model was constructed by intraperitoneal injection (i.p.) of 2.5 × 10 6 cells. Alongside positive control, two groups of EAC-bearing mice received 100 mg/kg of CS and GF nanoparticles/body weight daily for 14 days. The kidney function was conducted by measuring urea, creatinine, ions, (anti)/oxidative parameters and DNA damage. Also, measuring immunoreactivity of P53, proliferating cell nuclear antigen (PCNA), and B-cell lymphoma 2 (Bcl-2) and apoptosis protein. The outcomes illustrated notable kidney toxicity, which indicated by elevations in urea, creatinine, oxidative stress, DNA damage and induction of apoptosis. These events were supported by the drastic alteration in kidney structure through histological examination. Administration of CS and GF nanoparticles was able to enhance the antioxidant power, which further reduced oxidative damage, DNA injury, and apoptosis. These results indicated the protective and therapeutic role of biogenic chitosan and maitake nanoparticles against nephrotoxicity.
(© 2024 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society). Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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