Effect of experimental dentin etchants on dentin bond strength, metalloproteinase inhibition, and antibiofilm activity.

Autor: Sahadi BO; Department of Restorative Dentistry, Operative Dentistry Division, University of Campinas, Piracicaba Dental School, Avenida Limeira 901, Areião, 13414-903 Piracicaba, SP, Brazil. Electronic address: bia_sahadi@live.com., Sebold M; Department of Restorative Dentistry, Operative Dentistry Division, University of Campinas, Piracicaba Dental School, Avenida Limeira 901, Areião, 13414-903 Piracicaba, SP, Brazil. Electronic address: maiconsebold92@gmail.com., André CB; Department of Restorative Dentistry, Operative Dentistry Division, Federal University of Minas Gerais, School of Dentistry, Avenida Presidente Antonio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil. Electronic address: carolina.bosso@gmail.com., Nima G; Department of Biomedical Sciences, Ethics, Research and Education, Universidad de los Andes, School of Dentistry, Monseñor Álvaro del Portillo 12455, Las Condes 12455, Santiago, Chile. Electronic address: gabrieln_b@yahoo.com., Dos Santos A; Applied Chemistry Graduate Program, Midwest State University UNICENTRO, Alameda Élio Antônio Dalla Vechhia 838, Vila Carli, 85040-167 Guarapuava, PR, Brazil. Electronic address: andy_stos@hotmail.com., Chiari MDESC; Department of Molecular Biology, Federal University of São Paulo, São Paulo 04021-001, SP, Brazil. Electronic address: marina.chiari@unifesp.br., Nascimento FD; Department of Molecular Biology, Federal University of São Paulo, São Paulo 04021-001, SP, Brazil. Electronic address: fabio.dupart@unifesp.br., Tersariol ILDS; Department of Molecular Biology, Federal University of São Paulo, São Paulo 04021-001, SP, Brazil. Electronic address: ivarne.tersariol@gmail.com., Giannini M; Department of Restorative Dentistry, Operative Dentistry Division, University of Campinas, Piracicaba Dental School, Avenida Limeira 901, Areião, 13414-903 Piracicaba, SP, Brazil. Electronic address: gianinni@unicamp.br.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2024 Apr; Vol. 40 (4), pp. e12-e23. Date of Electronic Publication: 2024 Feb 17.
DOI: 10.1016/j.dental.2024.02.017
Abstrakt: Objective: this study evaluated dentin microtensile bond strength (µTBS) and failure modes (at 24 h and one year), bonding interface regarding hybridization, surface morphology regarding demineralization, in situ metalloproteinase (MMP) activity, and antibacterial effect of three dentin etchants compared to 35% phosphoric acid (PA).
Materials and Methods: The Adper Single Bond 2 adhesive (3 M Oral Care) was applied on moist dentin etched with PA (control) or on air-dried dentin etched with 3% aluminum nitrate + 2% oxalic acid (AN), 6.8% ferric oxalate + 10% citric acid (FO), or 10% citric acid (CA). The µTBS test used 40 human teeth (n = 10). Failure modes and surface morphology were analyzed by scanning electron microscopy (n = 3), while bonding interface morphology and MMP activity were evaluated by laser scanning confocal microscopy (n = 3). Antibacterial activity was evaluated against S. Mutans biofilm by means of viable cells count (CFU/mL).
Results: PA presented the highest bond strengths regardless of aging time. PA, AN, and CA showed stable bond strengths after one year of storage. Adhesive and mixed failures were predominant in all groups. Thin hybrid layers with short resin tags were observed for the experimental etchants. The AN-based etchant was able to inhibit MMP activity. All tested etchants presented antibacterial activity against S. Mutans biofilm.
Significance: This study suggests different dentin etchants capable of inhibiting MMP activity while also acting as cavity disinfectants.
Competing Interests: Declaration of Competing Interest The authors declare that no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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