The larvicidal effect of the supernatant of Lactobacillus acidophilus ATCC 4356 on Toxocara canis.

Autor: Netto de Oliveira da Cunha C; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil. Electronic address: carolnodacunha@gmail.com., Rodeghiero Collares S; Scientific Initiation Scholarship Holder. Laboratory of Parasitology, FAMED-FURG, Brazil., Carvalho Rodrigues D; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., Walcher DL; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., Quintana de Moura M; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., Rodrigues Martins LH; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., Baracy Klafke G; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., de Oliveira Arias JL; Integrated Analysis Center, School of Chemistry and Food, Federal University of Rio Grande, Av. Italia, Km 6 - Campus Carreiros, CEP 96203-900, Rio Grande, Rio Grande do Sul, Brazil., Carapelli R; School of Chemistry and Food, Federal University of Rio Grande, Avenida Itália, Km 08 - Campus Carreiros, CEP 96.203-900, Rio Grande, Rio Grande do Sul, Brazil., do Santos Espinelli Junior JB; School of Chemistry and Food, Federal University of Rio Grande, Avenida Itália, Km 08 - Campus Carreiros, CEP 96.203-900, Rio Grande, Rio Grande do Sul, Brazil., Scaini CJ; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil., Farias da Costa de Avila L; Faculty of Medicine (FAMED)/Parasitology Laboratory, Federal University of Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brazil.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2024 Mar; Vol. 258, pp. 108720. Date of Electronic Publication: 2024 Feb 16.
DOI: 10.1016/j.exppara.2024.108720
Abstrakt: Human toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with Lactobacillus acidophilus ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of Toxocara canis larvae in vitro. The present study aimed to investigate the characteristics of the CFS of L. acidophilus ATCC 4356, which may be involved in its larvicidal effects on T. canis. L. acidophilus ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quantified by high performance liquid chromatography (HPLC). The levels of pH and H 2 O 2 were also analyzed. To assess the larvicidal effect of the CFS, this was tested pure and diluted (1:2 to 1:128) on T. canis larvae. High concentrations of lactic and acetic acids were detected in the CFS. The acidity of the pure CFS was observed at pH 3.8, remaining acidic at dilutions of 1:2 to 1:16. Regarding the in vitro larvicidal effect, 100% death of T. canis larvae was observed using the pure CFS and 1:2 dilution. Based on these results, it can be inferred that the presence of higher concentrations of organic acids and low pH of the medium contributed to the larvicidal activity of the CFS of L. acidophilus ATCC 4356. In addition, the maintenance of the larvicidal effect, even after dilution, suggests a greater chance of the larvicidal effect of this CFS against T. canis in vivo.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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