Development of validated sandwich ELISA for detecting peanut allergen Ara h 3 in food.

Autor: Lin JF; Department of Food Science, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. Electronic address: ty0987359136@gmail.com., Chang KL; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: keeluch@kmu.edu.tw., Hsieh BS; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: hsiehbs@gmail.com., Hu YC; Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: huyujena@gmail.com., Huang ES; Department of Gastroenterology, Palo Alto Medical Foundation, Mountain View, CA 94040, USA. Electronic address: HuangE3@pamf.org., Yu HS; Department of Food Science, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. Electronic address: hsyu@mail.npust.edu.tw.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Jul 01; Vol. 445, pp. 138757. Date of Electronic Publication: 2024 Feb 15.
DOI: 10.1016/j.foodchem.2024.138757
Abstrakt: Peanut is an important food that can cause food allergies, often leading to moderate and severe allergic symptoms such as skin rashes, asthma, and even anaphylactic shock.Research indicates that Ara h 3 is one of the major peanut allergen. In order to establish a simple analytical method for detecting Ara h 3, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) with antibodies that were induced from purified Ara h 3. The experimental results showed that the purified Ara h 3 had good purity, and we successfully prepared capture and detection antibodies. The method established in this study exhibited high specificity and did not cross-react with soybeans, cashew nuts, and sesame. For validation, including precision, recovery and sensitivity were in good condition. We also detected the Ara h 3 in peanut related foods. Overall, the ELISA developed in this study is a reliable method for Ara h 3 detection.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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