Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber.

Autor:	Harris AG; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK. Electronic address: a.harris@bristol.ac.uk., Schot V; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK., Carrabba M; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK., Iacobazzi D; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK., Ghorbel MT; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK., Armstrong JPK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK., Perriman AW; School of Cellular and Molecular Medicine, Biomedical Sciences Building, University Walk, BS8 1TD Bristol, UK., Caputo M; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiac Surgery, University Hospitals Bristol, NHS Foundation Trust, BS2 8HW Bristol, UK., Biglino G; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiorespiratory Unit, Great Ormond Street Hospital for Children, NHS Foundation Trust, WC1N 3JH London, UK., Bartoli-Leonard F; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK. Electronic address: f.bartoli-leonard@bristol.ac.uk.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Mar 15; Vol. 5 (1), pp. 102899. Date of Electronic Publication: 2024 Feb 16.
DOI:	10.1016/j.xpro.2024.102899
Abstrakt:	Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold. For complete details on the use and execution of this protocol, please refer to Vafaee et al. 1 . Competing Interests: Declaration of interests J.P.K.A. is a co-founder and shareholder of Impulsonics Ltd., a company seeking to automate cell culture processes. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu