The multispecies microbial cluster of Fusobacterium, Parvimonas, Bacteroides and Faecalibacterium as a precision biomarker for colorectal cancer diagnosis.

Autor: Conde-Pérez K; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Aja-Macaya P; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Buetas E; Genomic and Health Department, FISABIO Foundation, Center for Advanced Research in Public Health, Valencia, Spain., Trigo-Tasende N; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Nasser-Ali M; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Rumbo-Feal S; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Nión P; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Arribas EM; Database Laboratory, Research Center for Information and Communication Technologies (CITIC), University of A Coruña (UDC), A Coruña, Spain., Estévez LS; Pathology Service and Biobank, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Otero-Alén B; Pathology Service and Biobank, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Noguera JF; Surgery Service, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Concha Á; Pathology Service and Biobank, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Pardiñas-López S; Periodontology and Oral Surgery, Pardiñas Medical Dental Clinic - Cell Therapy and Regenerative Medicine Group, Institute of Biomedical Research (INIBIC), A Coruña, Spain., Carda-Diéguez M; Genomic and Health Department, FISABIO Foundation, Center for Advanced Research in Public Health, Valencia, Spain., Gómez-Randulfe I; Medical Oncology Department, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Martínez-Lago N; Medical Oncology Department, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Ladra S; Database Laboratory, Research Center for Information and Communication Technologies (CITIC), University of A Coruña (UDC), A Coruña, Spain., Aparicio LMA; Medical Oncology Department, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Bou G; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Mira Á; Genomic and Health Department, FISABIO Foundation, Center for Advanced Research in Public Health, Valencia, Spain., Vallejo JA; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain., Poza M; Microbiome and Health Group (meiGAbiome), Microbiology Research Group, Institute of Biomedical Research (INIBIC) - Interdisciplinary Center for Chemistry and Biology (CICA) - University of A Coruña (UDC) - CIBER de Enfermedades Infecciosas (CIBERINFEC-ISCIII), Servicio de Microbiología, University Hospital of A Coruña (CHUAC), A Coruña, Spain.; Microbiome and Health Group, Faculty of Sciences, University of A Coruña (UDC), A Coruña, Spain.
Jazyk: angličtina
Zdroj: Molecular oncology [Mol Oncol] 2024 May; Vol. 18 (5), pp. 1093-1122. Date of Electronic Publication: 2024 Feb 17.
DOI: 10.1002/1878-0261.13604
Abstrakt: The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral and gut microbiomes of patients with CRC, as well as impaired gut vascular barrier function. In the present study, the microbiomes of saliva, crevicular fluid, feces, and non-neoplastic and tumor intestinal tissue samples of 93 CRC patients and 30 healthy individuals without digestive disorders (non-CRC) were analyzed by 16S rRNA metabarcoding procedures. The data revealed that Parvimonas, Fusobacterium, and Bacteroides fragilis were significantly over-represented in stool samples of CRC patients, whereas Faecalibacterium and Blautia were significantly over-abundant in the non-CRC group. Moreover, the tumor samples were enriched in well-known periodontal anaerobes, including Fusobacterium, Parvimonas, Peptostreptococcus, Porphyromonas, and Prevotella. Co-occurrence patterns of these oral microorganisms were observed in the subgingival pocket and in the tumor tissues of CRC patients, where they also correlated with other gut microbes, such as Hungatella. This study provides new evidence that oral pathobionts, normally located in subgingival pockets, can migrate to the colon and probably aggregate with aerobic bacteria, forming synergistic consortia. Furthermore, we suggest that the group composed of Fusobacterium, Parvimonas, Bacteroides, and Faecalibacterium could be used to design an excellent noninvasive fecal test for the early diagnosis of CRC. The combination of these four genera would significantly improve the reliability of a discriminatory test with respect to others that use a single species as a unique CRC biomarker.
(© 2024 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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