AMR Threat Perception Assessment of Heterotrophic Bacteria From Shrimp Aquaculture Through Epidemiological Cut off Values.

Autor:	Nadella RK; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Quality Assurance and Management Division, Willingdon Island, Matsyapuri P.O, Cochin, Kerala 682029, India., Panda SK; QA Advisor, Food Safety Standards Authority of India (FSSAI), FDA Bhawan, Kotla Rd near Bal Bhawan, New Delhi 110002, India., Kumar A; ICAR-Indian Institute of Wheat and Barley Research (ICAR-IIWBR), Karnal, Haryana 132001, India., Uchoi D; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Quality Assurance and Management Division, Willingdon Island, Matsyapuri P.O, Cochin, Kerala 682029, India., Kishore P; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Quality Assurance and Management Division, Willingdon Island, Matsyapuri P.O, Cochin, Kerala 682029, India., Badireddy MR; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Visakhapatnam Research Centre, Visakhapatnam, Andhra Pradesh 530003, India., Kuricheti PP; ICAR-Central Institute of Fisheries Education (ICAR-CIFE), Aquatic Environment and Health Management Division, Versova, Mumbai, Maharashtra 400061, India., Raman RP; ICAR-Central Institute of Fisheries Education (ICAR-CIFE), Aquatic Environment and Health Management Division, Versova, Mumbai, Maharashtra 400061, India., Mothadaka MP; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Visakhapatnam Research Centre, Visakhapatnam, Andhra Pradesh 530003, India.
Jazyk:	angličtina
Zdroj:	Journal of AOAC International [J AOAC Int] 2024 May 02; Vol. 107 (3), pp. 479-486.
DOI:	10.1093/jaoacint/qsae011
Abstrakt:	Background: Emergence and dissemination of antibiotic resistance is one of the major risks associated with the rampant usage of antibiotics in food-producing animals including aquaculture. Objective: To determine Epidemiological Cut-OFF (ECOFF) values of heterotrophic bacterial populations from shrimp culture environments against five different antibiotics. Methods: In this present study, bacterial samples were isolated from Penaeus vannamei culture environment in different locations of Andhra Pradesh, which is the aquaculture hub of India. The bacterial isolates were assessed for antibiotic resistance towards five antibiotics belonging to different classes (oxytetracycline, chloramphenicol, erythromycin, ciprofloxacin, and co-trimoxazole) by the disc diffusion method. Determination of Epidemiological Cut-OFF (ECOFF) values and analysis by employing normalized resistance interpretation (NRI) was carried out. Results: The most dominant bacterial populations from shrimp culture were Vibrio spp. (pathogenic bacteria) followed by Bacillus spp. (probiotic bacteria). The bacterial isolates showed highest resistance towards oxytetracycline (overall 23.38%) and in location L6 (59.4%) followed by co-trimoxazole (31.1%). ECOFF values calculated by employing NRI showed that the disc diffusion data were distributed in a normalized manner. The maximum ECOFF value was obtained for ciprofloxacin (23.32 mm), while the minimum value was observed for oxytetracycline (9.05 mm). The antibiotic resistant phenotypes showed that the majority of the heterotrophic bacterial isolates (>60%) belonged to the non-wild type phenotype and primarily towards oxytetracycline (90%). Conclusion: The presence of non-wild antibiotic-resistant phenotypes of heterotrophic bacterial populations (which include not only pathogenic bacteria but also probiotic bacteria) indicates that shrimp culture ponds may be a reservoir for drug-resistant bacteria and there is a greater risk associated with transmission of resistant genes across bacterial flora. Highlights: NRI analysis of antibiotic disc diffusion data of heterotrophic bacterial populations in shrimp aquaculture environments revealed that majority of them belonged to non-wild type (90%) paticularly to oxytetracycline in comparison to other studied antibiotics (chloramphenicol, erythromycin, ciprofloxacin and co-trimoxazole). (© The Author(s) 2024. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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