Orchid seeds are not always short lived in a conventional seed bank!

Autor:	Francisqueti AM; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil., Marin RR; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil., Hengling MM; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil., Hosomi ST; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil., Pritchard HW; Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, PR China.; Royal Botanic Gardens, Kew, Wakehurst, Ardingly, West Sussex RH17 6TN, UK., Custódio CC; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil., Machado-Neto NB; Unoeste Campus II, Agronomy College, Building 2, room 201, Rodovia Raposo Tavares, km 572. Limoeiro, Presidente Prudente SP, 19067-175, Brazil.
Jazyk:	angličtina
Zdroj:	Annals of botany [Ann Bot] 2024 May 13; Vol. 133 (7), pp. 941-952.
DOI:	10.1093/aob/mcae021
Abstrakt:	Background and Aims: Orchid seeds are reputed to be short lived in dry, cold storage conditions, potentially limiting the use of conventional seed banks for long-term ex situ conservation. This work explores whether Cattleya seeds are long lived or not during conventional storage (predried to ~12 % relative humidity, then stored at -18 °C). Methods: We explored the possible interaction of factors influencing seed lifespan in eight species of the genus Cattleya using physiological (germination and vigour), biochemical (gas chromatography), biophysical (differential scanning calorimetry) and morphometric methods. Seeds were desiccated to ~3 % moisture content and stored at -18 °C for more than a decade, and seed quality was measured via three in vitro germination techniques. Tetrazolium staining was also used to monitor seed viability during storage. The morphometric and germination data were subjected to ANOVA and cluster analysis, and seed lifespan was subjected to probit analysis. Key Results: Seeds of all Cattleya species were found to be desiccation tolerant, with predicted storage lifespans (P50y) of ~30 years for six species and much longer for two species. Cluster analysis showed that the three species with the longest-lived seeds had smaller (9-11 %) airspaces around the embryo. The post-storage germination method impacted the quality assessment; seeds equilibrated at room temperature for 24 h or in 10 % sucrose solution had improved germination, particularly for the seeds with the smallest embryos. Chromatography revealed that the seeds of all eight species were rich in linoleic acid, and differential scanning calorimetry identified a peak that might be auxiliary to selecting long-lived seeds. Conclusions: These findings show that not all orchids produce seeds that are short lived, and our trait analyses might help to strengthen prediction of seed longevity in diverse orchid species. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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