Nitrate-driven anaerobic oxidation of ethane and butane by bacteria.

Autor:	Wu M; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia., Li J; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia., Lai CY; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia.; College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China., Leu AO; Centre for Microbiome Research, School of Biomedical Sciences, Translational Research Institute, Queensland University of Technology (QUT), Woolloongabba, Queensland, Australia., Sun S; Computational Science Program, The University of Texas at El Paso, El Paso, TX, United States., Gu R; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia., Erler DV; Faculty of Science and Engineering, Southern Cross University, Lismore, New South Wales, Australia., Liu L; Metabolomics Australia (Queensland Node), Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland 4072, Australia., Li L; Department of Physics, University of Texas at El Paso, El Paso, TX, United States., Tyson GW; Centre for Microbiome Research, School of Biomedical Sciences, Translational Research Institute, Queensland University of Technology (QUT), Woolloongabba, Queensland, Australia., Yuan Z; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia.; School of Energy and Environment, City University of Hong Kong, Hong Kong SAR, China., McIlroy SJ; Centre for Microbiome Research, School of Biomedical Sciences, Translational Research Institute, Queensland University of Technology (QUT), Woolloongabba, Queensland, Australia., Guo J; Australian Centre for Water and Environmental Biotechnology, Faculty of Engineering, Architecture and Information Technology, The University of Queensland, St Lucia, Queensland 4072, Australia.
Jazyk:	angličtina
Zdroj:	The ISME journal [ISME J] 2024 Jan 08; Vol. 18 (1).
DOI:	10.1093/ismejo/wrad011
Abstrakt:	The short-chain gaseous alkanes (ethane, propane, and butane; SCGAs) are important components of natural gas, yet their fate in environmental systems is poorly understood. Microbially mediated anaerobic oxidation of SCGAs coupled to nitrate reduction has been demonstrated for propane, but is yet to be shown for ethane or butane-despite being energetically feasible. Here we report two independent bacterial enrichments performing anaerobic ethane and butane oxidation, respectively, coupled to nitrate reduction to dinitrogen gas and ammonium. Isotopic 13C- and 15N-labelling experiments, mass and electron balance tests, and metabolite and meta-omics analyses collectively reveal that the recently described propane-oxidizing "Candidatus Alkanivorans nitratireducens" was also responsible for nitrate-dependent anaerobic oxidation of the SCGAs in both these enrichments. The complete genome of this species encodes alkylsuccinate synthase genes for the activation of ethane/butane via fumarate addition. Further substrate range tests confirm that "Ca. A. nitratireducens" is metabolically versatile, being able to degrade ethane, propane, and butane under anoxic conditions. Moreover, our study proves nitrate as an additional electron sink for ethane and butane in anaerobic environments, and for the first time demonstrates the use of the fumarate addition pathway in anaerobic ethane oxidation. These findings contribute to our understanding of microbial metabolism of SCGAs in anaerobic environments. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu