The involvement of cytokine gene polymorphism in determining the vulnerability to Blastocystis and Helicobacter pylori co-infection in the Egyptian population.

Autor: Ibrahim A; Genetic engineering and Biotechnology research institute, University of Sadat City (GEBRI, USC), Egypt; Departments of Medical Parasitology, Faculty of Medicine, Cairo University (Laboratory of Molecular Medical Parasitology, LMMP), Egypt. Electronic address: chemistasmaain@gmail.com., Kamel NO; Department of Medical Parasitology, Faculty of Medicine, October 6 University, Egypt., Rageh F; Infectious disease, Gastroenterology and hepatology department, Suez University, Egypt., Elgamal R; Clinical pathology department, Faculty of Medicine, Suez University, Egypt., Mansour Salama B; Infectious and Endemic Diseases Department, Faculty of Medicine Suez Canal University, Egypt., Sakr MA; Medical Microbiology and Immunology Department, Faculty of Medicine, Suez University, P.O. Box:43221, Suez, Egypt., Elhoseeny MM; Internal Medicine Department, Faculty of Medicine, Suez University, Egypt., Osman EM; Immunology and allergy department, Medical Research Institute Alexandria University, Egypt., Sayed S; Community Medicine Faculty of Medicine, Suez University, Egypt., Ramadan ME; Department of Medical Parasitology, Faculty of Medicine, Suez University, P.O. Box:43221, Suez, Egypt.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Apr; Vol. 252, pp. 107137. Date of Electronic Publication: 2024 Feb 15.
DOI: 10.1016/j.actatropica.2024.107137
Abstrakt: Aims: The present study aimed to identify any potential association between IL-1β and TNF-α gene polymorphism and the risk of Blastocystis infection as well as co-infection of Blastocystis with Helicobacter pylori (H.pylori).
Methodology: A total of 314 stool samples were collected and examined microscopically for the detection of parasitic infection. DNA was extracted from all samples and utilized to identify Blastocystis molecularly. Positive samples were used for H. pylori detection by rapid tests and PCR. Moreover, we investigate polymorphism in the TNF-α gene at position -1031T/C, -308 G/A, and IL-1β at position +3954C/T using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
Results: Out of the 314 stool samples, Blastocystis was detected in 93 (29.6 %); among them, 54 (58.1 %) had a mixed infection of Blastocystis with H. pylori. The TT genotype of the IL-1β gene at position +3954 was significantly higher in Blasocystis-infected patients than in uninfected patients (17.2% vs. 6.3 %, P = 0.02), which might be considered a risk factor (OR = 3.2; CI =1.21-8.52). The TNF-α at position -1031 TT genotype was significantly higher in Blastocystis-infected patients than uninfected patients (44.1% vs. 10.8 %, P< 0.0001). The T allele (OR= 2.67; CI=1.51-4.72, P = 0.0008) might be considered a risk factor. The TNF- α at position -308 AA genotype is higher in Blasocystis infected than uninfected (17.2% vs 7.2 %, P = 0.03). TNF-α -308 AA (OR = 2.72; CI = 1.08-6.89) and A allele (OR= 1.46; CI= 0.797-2.66) might be considered risk factors. The TNF- α at position -308 G/A showed that the GG is the most frequent genotype in Blastocystis with H. pylori-positive patients with a significant association (P = 0.004), as well as the G allele (P = 0.02). The G allele (OR=1.924; CI= 1.071-3.454) might be considered a risk factor for co-infection of Blastocystis and H. pylori.
Conclusion: SNPs (-1031 T/C and -308 G/A) of the TNF-α and (+3954 C/T) of the IL-1β may be a useful marker in the assessment of the risk of Blastocystis infection, and TNF-α at position -308 G/A) may be a predictor for co-infection of Blastocystis with H. pylori.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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