Design and characterization of an electrochemically-modulated membrane chromatography device.

Autor: Röcker D; Chair of Bioseparation Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, Garching 85748, Germany; Munich Institute for Integrated Materials, Energy and Process Engineering, Technical University of Munich, Lichtenbergstraße 4a, Garching 85748, Germany., Dietmann K; Chair of Bioseparation Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, Garching 85748, Germany., Nägler L; Chair of Bioseparation Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, Garching 85748, Germany., Su X; Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, United States., Fraga-García P; Chair of Bioseparation Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, Garching 85748, Germany., Schwaminger SP; Division of Medicinal Chemistry, Otto-Loewi Research Center, Medical University of Graz, Neue Stiftingtalstraße 6, Graz 8010, Austria; BioTechMed-Graz, Mozartgasse 12/II, Graz 8010, Austria. Electronic address: sebastian.schwaminger@medunigraz.at., Berensmeier S; Chair of Bioseparation Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, Garching 85748, Germany; Munich Institute for Integrated Materials, Energy and Process Engineering, Technical University of Munich, Lichtenbergstraße 4a, Garching 85748, Germany. Electronic address: s.berensmeier@tum.de.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Mar 15; Vol. 1718, pp. 464733. Date of Electronic Publication: 2024 Feb 10.
DOI: 10.1016/j.chroma.2024.464733
Abstrakt: Membrane separations offer a compelling alternative to traditional chromatographic methods by overcoming mass transport limitations. We introduce an additional degree of freedom in modulating membrane chromatography by using metalized membranes in a potential-driven process. Investigating the impact of a gold coating on membrane characteristics, the sputtered gold layer enhances the surface conductivity with stable electrochemical behavior. However, this comes at the expense of reduced permeability, wettability, and static binding capacity (∼ 474 µg g -1 of maleic acid). The designed device displayed a homogenous flow distribution, and the membrane electrodes exhibit predominantly capacitive behavior during potential application. Modulating the electrical potential during the adsorption and desorption phase strongly influenced the binding and elution behavior of anion-exchange membranes. Switching potentials between ±1.0 V vs. Ag/AgCl induces desorption, confirming the process principle. Elution efficiency reaches up to 58 % at -1.0 V vs. Ag/AgCl in the desorption phase without any alteration of the mobile phase. Increasing the potential perturbation ranging from +1.0 V to -1.0 V vs. Ag/AgCl resulted in reduced peak width and improved elution behavior, demonstrating the feasibility of electrochemically-modulated membrane chromatography. The developed process has great potential as a gentle and sustainable separation step in the biotechnological and chemical industry.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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