Small-angle X-ray scattering unveils the internal structure of lipid nanoparticles.

Autor: Spinozzi F; Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy. Electronic address: f.spinozzi@univpm.it., Moretti P; Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy., Perinelli DR; School of Pharmacy, University of Camerino, Camerino, Italy., Corucci G; Institut Laue-Langevin, Grenoble, France; École Doctorale de Physique, Université Grenoble Alpes, Saint-Martin-d'Héres, France; Department of Chemistry, Imperial College London, London, UK., Piergiovanni P; Department of Life and Environmental Sciences, Polytechnic University of Marche, Italy., Amenitsch H; Institute for Inorganic Chemistry, Graz University of Technology, Graz, Austria., Sancini GA; School of Medicine and Surgery, University of Milan Bicocca, Milan, Italy., Franzese G; Secció de Física Estadística i Interdisciplinària, Departament de Física de la Matèria Condensada, & Institut de Nanociència i Nanotecnologia, Universitat de Barcelona, Martí i Franquès 1, Barcelona, 08028, Spain., Blasi P; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. Electronic address: p.blasi@unibo.it.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2024 May 15; Vol. 662, pp. 446-459. Date of Electronic Publication: 2024 Feb 12.
DOI: 10.1016/j.jcis.2024.02.076
Abstrakt: Lipid nanoparticles own a remarkable potential in nanomedicine, only partially disclosed. While the clinical use of liposomes and cationic lipid-nucleic acid complexes is well-established, liquid lipid nanoparticles (nanoemulsions), solid lipid nanoparticles, and nanostructured lipid carriers have even greater possibilities. However, they face obstacles in being used in clinics due to a lack of understanding about the molecular mechanisms controlling their drug loading and release, interactions with the biological environment (such as the protein corona), and shelf-life stability. To create effective drug delivery carriers and successfully translate bench research to clinical settings, it is crucial to have a thorough understanding of the internal structure of lipid nanoparticles. Through synchrotron small-angle X-ray scattering experiments, we determined the spatial distribution and internal structure of the nanoparticles' lipid, surfactant, and the bound water in them. The nanoparticles themselves have a barrel-like shape that consists of coplanar lipid platelets (specifically cetyl palmitate) that are covered by loosely spaced polysorbate 80 surfactant molecules, whose polar heads retain a large amount of bound water. To reduce the interface cost of bound water with unbound water without stacking, the platelets collapse onto each other. This internal structure challenges the classical core-shell model typically used to describe solid lipid nanoparticles and could play a significant role in drug loading and release, biological fluid interaction, and nanoparticle stability, making our findings valuable for the rational design of lipid-based nanoparticles.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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