Intestinal lysozyme1 deficiency alters microbiota composition and impacts host metabolism through the emergence of NAD + -secreting ASTB Qing110 bacteria.

Autor:	Zhang C; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China., Xiang C; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China., Zhou K; Beijing Institute of Genomics, Chinese Academy of Sciences, China National Center for Bioinformation, Beijing, China.; University of Chinese Academy of Sciences, Beijing, China., Liu X; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China.; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China., Qiao G; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China., Zhao Y; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China., Dong K; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China.; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China., Sun K; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China., Liu Z; Institute for Immunology, School of Medicine, Tsinghua University, Beijing, China.; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Jazyk:	angličtina
Zdroj:	MSystems [mSystems] 2024 Mar 19; Vol. 9 (3), pp. e0121423. Date of Electronic Publication: 2024 Feb 16.
DOI:	10.1128/msystems.01214-23
Abstrakt:	The intestine plays a pivotal role in nutrient absorption and host defense against pathogens, orchestrated in part by antimicrobial peptides secreted by Paneth cells. Among these peptides, lysozyme has multifaceted functions beyond its bactericidal activity. Here, we uncover the intricate relationship between intestinal lysozyme, the gut microbiota, and host metabolism. Lysozyme deficiency in mice led to altered body weight, energy expenditure, and substrate utilization, particularly on a high-fat diet. Interestingly, these metabolic benefits were linked to changes in the gut microbiota composition. Cohousing experiments revealed that the metabolic effects of lysozyme deficiency were microbiota-dependent. 16S rDNA sequencing highlighted differences in microbial communities, with ASTB_g (OTU60) highly enriched in lysozyme knockout mice. Subsequently, a novel bacterium, ASTB Qing110 , corresponding to ASTB_g (OTU60), was isolated. Metabolomic analysis revealed that ASTB Qing110 secreted high levels of NAD + , potentially influencing host metabolism. This study sheds light on the complex interplay between intestinal lysozyme, the gut microbiota, and host metabolism, uncovering the potential role of ASTB Qing110 as a key player in modulating metabolic outcomes. Importance: The impact of intestinal lumen lysozyme on intestinal health is complex, arising from its multifaceted interactions with the gut microbiota. Lysozyme can both mitigate and worsen certain health conditions, varying with different scenarios. This underscores the necessity of identifying the specific bacterial responses elicited by lysozyme and understanding their molecular foundations. Our research reveals that a deficiency in intestinal lysozyme1 may offer protection against diet-induced obesity by altering bacterial populations. We discovered a strain of bacterium, ASTB Qing110 , which secretes NAD + and is predominantly found in lyz1-deficient mice. Qing110 demonstrates positive effects in both C. elegans and mouse models of ataxia telangiectasia. This study sheds light on the intricate role of lysozyme in influencing intestinal health. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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