Polyphenol exposure of mothers and infants assessed by LC-MS/MS based biomonitoring in breast milk.

Autor: Berger S; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria., Oesterle I; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria.; Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie, 1090, Wien, Austria.; Vienna Doctoral School of Chemistry (DoSChem), University of Vienna, 1090, Vienna, Austria., Ayeni KI; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria.; Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria., Ezekiel CN; Institute for Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad-Lorenz Str. 20, 3430, Tulln, Austria., Rompel A; Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie, 1090, Wien, Austria., Warth B; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria. benedikt.warth@univie.ac.at.; Exposome Austria, Research Infrastructure and National EIRENE Node, Vienna, Austria. benedikt.warth@univie.ac.at.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Mar; Vol. 416 (7), pp. 1759-1774. Date of Electronic Publication: 2024 Feb 16.
DOI: 10.1007/s00216-024-05179-y
Abstrakt: Exposure to polyphenols is relevant throughout critical windows of infant development, including the breastfeeding phase. However, the quantitative assessment of polyphenols in human breast milk has received limited attention so far, though polyphenols may positively influence infant health. Therefore, a targeted LC-MS/MS assay was developed to investigate 86 analytes representing different polyphenol classes in human breast milk. The sample preparation consisted of liquid extraction, salting out, freeze-out, and a dilution step. Overall, nearly 70% of the chemically diverse polyphenols fulfilled all strict validation criteria for full quantitative assessment. The remaining analytes did not fulfill all criteria at every concentration level, but can still provide useful semi-quantitative insights into nutritional and biomedical research questions. The limits of detection for all analyzed polyphenols were in the range of 0.0041-87 ng*mL -1 , with a median of 0.17 ng*mL -1 . Moreover, the mean recovery was determined to be 82% and the mean signal suppression and enhancement effect was 117%. The developed assay was applied in a proof-of-principle study to investigate polyphenols in breast milk samples provided by twelve Nigerian mothers at three distinct time points post-delivery. In total, 50 polyphenol analytes were detected with almost half being phenolic acids. Phase II metabolites, including genistein-7-β-D-glucuronide, genistein-7-sulfate, and daidzein-7-β-D-glucuronide, were also detected in several samples. In conclusion, the developed method was demonstrated to be fit-for-purpose to simultaneously (semi-) quantify a wide variety of polyphenols in breast milk. It also demonstrated that various polyphenols including their biotransformation products were present in breast milk and therefore likely transferred to infants where they might impact microbiome development and infant health.
(© 2024. The Author(s).)
Databáze: MEDLINE
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