From imaging to precision: low cost and accurate determination of stereotactic coordinates for brain surgery Sapajus apella using MRI.

Autor:	Pedrosa LRR; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil., Leal LCP; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; National Primate Center, Institute Evandro Chagas, Ananindeua, Brazil., Muniz JAPC; National Primate Center, Institute Evandro Chagas, Ananindeua, Brazil., Bastos CO; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil., Gomes BD; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil., Krejcová LV; Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroscience [Front Neurosci] 2024 Feb 01; Vol. 18, pp. 1324669. Date of Electronic Publication: 2024 Feb 01 (Print Publication: 2024).
DOI:	10.3389/fnins.2024.1324669
Abstrakt:	The capuchin monkey ( Sapajus apella ), a New World monkey species, exhibits prominent characteristics that make it an ideal model for neuroscience research. These characteristics include its phylogenetic traits, telencephalization coefficient, anatomical structures and pathways, genetic profile, immune responses, cognitive abilities, and complex behavioral repertoires. Traditionally, methodologies for stereotactic neurosurgery in research models have relied on the use of brain atlases. However, this approach can lead to errors due to the considerable variation in brain size and shape among individual monkeys. To address this issue, we developed a protocol for deriving individual coordinates for each monkey using a straightforward and relatively inexpensive method involving MRI imaging. Our protocol utilizes a specially designed, 3D-printed stereotactic head-holder that is safe to use with an MR magnet, non-invasive placement of fiducial markers, and post-processing with open-source software. This approach enhances MRI data visualization, improves anatomical targeting, and refines the design of neurosurgical experiments. Our technique could also prove beneficial in other areas of neuroscience research that require accurate calculation of stereotaxic coordinates. Furthermore, it could be useful for other nonhuman primate species for which brain atlases are typically unavailable. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Pedrosa, Leal, Muniz, Bastos, Gomes and Krejcová.)
Databáze:	MEDLINE
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