Prognostic value of the expression and localization of cell proliferation and apoptosis markers in unicystic ameloblastomas.

Autor: Vieira Costa R; Laboratory of Pathological Anatomy and Immunohistochemistry, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil., Balbinot KM; Laboratory of Pathological Anatomy and Immunohistochemistry, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil., da Silveira GCAR; Laboratory of Pathological Anatomy and Immunohistochemistry, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil., Kataoka MSDS; Cell Culture Laboratory, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil., de Menezes SAF; Department of Periodontics, University Center of Pará, Belém, PA, Brazil., Freitas VM; Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Vasconcelos ACU; Center for the Diagnosis of Diseases of the Mouth, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457/607, Pelotas, Rio Grande do Sul, 96015-560, Brazil., Etges A; Center for the Diagnosis of Diseases of the Mouth, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457/607, Pelotas, Rio Grande do Sul, 96015-560, Brazil., Martins Montalli VA; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua Dr. José Rocha Junqueira, 13-Pte. Preta, Campinas, SP, 13045-755, Brazil., Santos FP; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua Dr. José Rocha Junqueira, 13-Pte. Preta, Campinas, SP, 13045-755, Brazil., Alves Júnior SM; Laboratory of Pathological Anatomy and Immunohistochemistry, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil., Jaeger RG; Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Pinheiro JJV; Cell Culture Laboratory, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil. radface@hotmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 15; Vol. 14 (1), pp. 3856. Date of Electronic Publication: 2024 Feb 15.
DOI: 10.1038/s41598-024-54132-7
Abstrakt: The aim of this study was to verify whether the expression of cell proliferation and apoptosis markers in different types of unicystic ameloblastoma (UA) is associated with the location of neoplastic cells. Immunohistochemical study with a sample of 32 cases of UA, 11 cases of conventional ameloblastoma (CAM) and ten dental follicles (DF) cases was performed. Cell proliferation was assessed using Ki-67 status, and apoptosis by caspase-3 expression. Mural UA (MUA) showed a higher immunostaining of Ki-67 (p < 0.05) and a lower immunostaining of Caspase-3 (p < 0.05) compared with luminal and intraluminal subtypes of UA and CAM. The neoplastic cells of the MUA's cystic capsule showed a higher expression of Ki-67 protein (p < 0.0001) and a lower expression of Caspase-3 (p < 0.0001) compared with the lumen. DF showed lower Ki-67 and Caspase-3 immunostaining (p < 0.05) than neoplasms. The higher immunoexpression of Ki-67 and the lower immunoexpression of Caspase-3 in MUA, in the parenchyma cells within the cystic capsule, suggest an association between the biological behaviour and location of neoplastic cells in a tumour.
(© 2024. The Author(s).)
Databáze: MEDLINE
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