Compressive stress triggers fibroblasts spreading over cancer cells to generate carcinoma in situ organization.

Autor: Bertillot F; Institut Curie, PSL Research University, CNRS UMR 144, F-75005, Paris, France.; Department of Cell and Tissue Dynamics, Max Planck Institute for Molecular Biomedicine, 48149, Münster, Germany., Andrique L; LP2N, Laboratoire Photonique Numérique et Nanosciences, Univ. Bordeaux, F-33400, Talence, France.; Institut d'Optique Graduate School & CNRS UMR 5298, F-33400, Talence, France.; VoxCell, TBM-Core, CNRS UMS 3427 & INSERM US 005, Univ. Bordeaux, F-33000, Bordeaux, France., Ureña Martin C; Institut Curie, PSL Research University, CNRS UMR3666-INSERM U1143, F-75005, Paris, France., Zajac O; Institut Curie, PSL Research University, CNRS UMR 144, F-75005, Paris, France., de Plater L; Institut Curie, PSL Research University, U934/UMR3215, F-75005, Paris, France., Norton MM; VoxCell, TBM-Core, CNRS UMS 3427 & INSERM US 005, Univ. Bordeaux, F-33000, Bordeaux, France., Richard A; LP2N, Laboratoire Photonique Numérique et Nanosciences, Univ. Bordeaux, F-33400, Talence, France.; Institut d'Optique Graduate School & CNRS UMR 5298, F-33400, Talence, France.; VoxCell, TBM-Core, CNRS UMS 3427 & INSERM US 005, Univ. Bordeaux, F-33000, Bordeaux, France., Alessandri K; Institut Curie, PSL Research University, CNRS UMR 144, F-75005, Paris, France., Gurchenkov BG; Institut Curie, PSL Research University, CNRS UMR 144, F-75005, Paris, France., Fage F; Laboratoire Matière et Systèmes Complexes, Université Paris Cité, CNRS UMR7057, F-75013, Paris, France., Asnacios A; Laboratoire Matière et Systèmes Complexes, Université Paris Cité, CNRS UMR7057, F-75013, Paris, France., Lamaze C; Institut Curie, PSL Research University, CNRS UMR3666-INSERM U1143, F-75005, Paris, France., Das M; Rochester Institute of Technology, Rochester, NY, USA., Maître JL; Institut Curie, PSL Research University, U934/UMR3215, F-75005, Paris, France., Nassoy P; LP2N, Laboratoire Photonique Numérique et Nanosciences, Univ. Bordeaux, F-33400, Talence, France. pierre.nassoy@u-bordeaux.fr.; Institut d'Optique Graduate School & CNRS UMR 5298, F-33400, Talence, France. pierre.nassoy@u-bordeaux.fr., Matic Vignjevic D; Institut Curie, PSL Research University, CNRS UMR 144, F-75005, Paris, France. danijela.vignjevic@curie.fr.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Feb 15; Vol. 7 (1), pp. 184. Date of Electronic Publication: 2024 Feb 15.
DOI: 10.1038/s42003-024-05883-6
Abstrakt: At the early stage of tumor progression, fibroblasts are located at the outer edges of the tumor, forming an encasing layer around it. In this work, we have developed a 3D in vitro model where fibroblasts' layout resembles the structure seen in carcinoma in situ. We use a microfluidic encapsulation technology to co-culture fibroblasts and cancer cells within hollow, permeable, and elastic alginate shells. We find that in the absence of spatial constraint, fibroblasts and cancer cells do not mix but segregate into distinct aggregates composed of individual cell types. However, upon confinement, fibroblasts enwrap cancer cell spheroid. Using a combination of biophysical methods and live imaging, we find that buildup of compressive stress is required to induce fibroblasts spreading over the aggregates of tumor cells. We propose that compressive stress generated by the tumor growth might be a mechanism that prompts fibroblasts to form a capsule around the tumor.
(© 2024. The Author(s).)
Databáze: MEDLINE
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