| Autor: |
Steves MA; Department of Chemistry, University of California, Berkeley, California, USA; email: xuk@berkeley.edu., He C; Department of Chemistry, University of California, Berkeley, California, USA; email: xuk@berkeley.edu., Xu K; Department of Chemistry, University of California, Berkeley, California, USA; email: xuk@berkeley.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Annual review of physical chemistry [Annu Rev Phys Chem] 2024 Jun; Vol. 75 (1), pp. 163-183. Date of Electronic Publication: 2024 Jun 14. |
| DOI: |
10.1146/annurev-physchem-070623-034225 |
| Abstrakt: |
By superlocalizing the positions of millions of single molecules over many camera frames, a class of super-resolution fluorescence microscopy methods known as single-molecule localization microscopy (SMLM) has revolutionized how we understand subcellular structures over the past decade. In this review, we highlight emerging studies that transcend the outstanding structural (shape) information offered by SMLM to extract and map physicochemical parameters in living mammalian cells at single-molecule and super-resolution levels. By encoding/decoding high-dimensional information-such as emission and excitation spectra, motion, polarization, fluorescence lifetime, and beyond-for every molecule, and mass accumulating these measurements for millions of molecules, such multidimensional and multifunctional super-resolution approaches open new windows into intracellular architectures and dynamics, as well as their underlying biophysical rules, far beyond the diffraction limit. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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