Metagenomic analysis unravels novel taxonomic differences in the uterine microbiome between healthy mares and mares with endometritis.

Autor: Virendra A; Department of Animal Reproduction, Gynecology and Obstetrics, Mumbai Veterinary College, Mumbai, Maharashtra, India., Gulavane SU; Department of Animal Reproduction, Gynecology and Obstetrics, Mumbai Veterinary College, Mumbai, Maharashtra, India., Ahmed ZA; Equus Stud Farm, Pune, Maharashtra, India., Reddy R; Nanoli Stud and Agricultural Farm, Pune, Maharashtra, India., Chaudhari RJ; Department of Animal Reproduction, Gynecology and Obstetrics, Mumbai Veterinary College, Mumbai, Maharashtra, India., Gaikwad SM; Department of Animal Reproduction, Gynecology and Obstetrics, Mumbai Veterinary College, Mumbai, Maharashtra, India., Shelar RR; Department of Animal Reproduction, Gynecology and Obstetrics, Mumbai Veterinary College, Mumbai, Maharashtra, India., Ingole SD; Department of Veterinary Physiology, Mumbai Veterinary College, Mumbai, Maharashtra, India., Thorat VD; Department of Veterinary Microbiology, Mumbai Veterinary College, Mumbai, Maharashtra, India., Khanam A; Department of Large Animal Medicine and Surgery, School of Veterinary Medicine, St. George's University, Grenada, West Indies., Khan FA; Department of Large Animal Medicine and Surgery, School of Veterinary Medicine, St. George's University, Grenada, West Indies.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2024 Mar; Vol. 10 (2), pp. e1369.
DOI: 10.1002/vms3.1369
Abstrakt: Background: The application of high throughput technologies has enabled unravelling of unique differences between healthy mares and mares with endometritis at transcriptomic and proteomic levels. However, differences in the uterine microbiome are yet to be investigated.
Objectives: The present study was aimed at evaluating the differences in uterine microbiome between healthy mares and mares with endometritis.
Methods: Low-volume lavage (LVL) samples were collected from the uterus of 30 mares classified into healthy (n = 15) and endometritis (n = 15) based on their reproductive history, intrauterine fluid accumulation, gross appearance of LVL samples, endometrial cytology and bacterial culture. The samples were subjected to 16S rRNA sequencing.
Results: Notable differences in the uterine microbiome were observed between healthy mares and mares with endometritis at various taxonomic levels. In healthy mares, the most abundant phylum, class, order and family were Firmicutes, Bacilli, Bacillales and Paenibacillaceae, respectively. In contrast, the most abundant corresponding taxonomic levels in mares with endometritis were Proteobacteria, Gammaproteobacteria, Enterobacterales and Enterobacteriaceae, respectively. At the genus level, Brevibacillus and Paenibacillus were more abundant in healthy mares, whereas Escherichia, Salmonella and Klebsiella were more abundant in mares with endometritis. In healthy mares, Brevibacillus brevis was the most abundant species, followed by Brevibacillus choshinensis and Paenibacillus sp JDR-2. However, in mares with endometritis, Escherichia coli was the most abundant species, followed by Salmonella enterica and Klebsiella pneumoniae.
Conclusions: These results confirmed the previously reported presence of a uterine microbiome in healthy mares and helped unravel some alterations that occur in mares with endometritis. The findings can potentially help formulate new approaches to prevent or treat equine endometritis.
(© 2024 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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