Tandem mass spectrometry using continuous-wave infrared multiphoton dissociation in an electrostatic linear ion trap.
Autor: | Carrick IJ; Department of Chemistry, Purdue University, West Lafayette, Indiana, USA., Fabijanczuk KC; Department of Chemistry, Purdue University, West Lafayette, Indiana, USA., Rong J; Department of Chemistry, Purdue University, West Lafayette, Indiana, USA., McLuckey SA; Department of Chemistry, Purdue University, West Lafayette, Indiana, USA. |
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Jazyk: | angličtina |
Zdroj: | Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2024 Mar 30; Vol. 38 (6), pp. e9698. |
DOI: | 10.1002/rcm.9698 |
Abstrakt: | Rationale: The electrostatic linear ion trap (ELIT) can be operated as a multi-reflection time-of-flight (MR-TOF) or Fourier transform (FT) mass analyzer. It has been shown to be capable of performing high-resolution mass analysis and high-resolution ion isolations. Although it has been used in charge-detection mass spectrometry (CDMS), it has not been widely used as a conventional mass spectrometer for ensemble measurements of ions, or for tandem mass spectrometer. The advantages of tandem mass spectrometer with high-resolution ion isolations in the ELIT have thus not been fully exploited. Methods: A homebuilt ELIT was modified with BaF Results: Due to the low energy absorption of gas-phase ions during each oscillation in the ELIT, fragmentation was found to occur over a range of oscillations in the ELIT generating a homogeneous ion beam. A mirror-switching pulse is shown to create time-varying perturbations in this beam that oscillate at the fragment ion characteristic frequencies and generate a time-domain signal. This was found to recover FT signal for protonated pYGGFL and pSGGFL precursor ions. Conclusions: Fragmentation at the turnaround point of an ELIT by continuous-wave infrared multiphoton dissociation (cw-IRMPD) is demonstrated. In cases where laser power absorption is low and fragmentation occurs over many laps, a mirror-switching pulse may be used to recover varying time-domain signal. The combination of laser activation at the turnaround points and mirror-switching isolation allows for tandem MS in the ELIT. (© 2024 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd.) |
Databáze: | MEDLINE |
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