Applying mechanical forces on Drosophila tissues in vivo using the StretchCo, a 3D-printable device.

Autor:	Gracia M; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Polarity, Division and Morphogenesis lab, 75005 Paris, France., Lefèvre BM; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Stem Cells and Tissue Homeostasis lab, 75005 Paris, France., Güell Alonso R; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Stem Cells and Tissue Homeostasis lab, 75005 Paris, France., Cachoux V; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Polarity, Division and Morphogenesis lab, 75005 Paris, France., Balakireva M; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Polarity, Division and Morphogenesis lab, 75005 Paris, France., Guirao B; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Polarity, Division and Morphogenesis lab, 75005 Paris, France., Cuvelier D; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR144, Cell Biology and Cancer Department, System Biology of Cell Polarity and Cell Division lab, 75005 Paris, France., Bardin AJ; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Stem Cells and Tissue Homeostasis lab, 75005 Paris, France. Electronic address: allison.bardin@curie.fr., Bellaïche Y; Institut Curie, Université PSL, Sorbonne Université, CNRS UMR3215, INSERM U934, Genetics and Developmental Biology Department, Polarity, Division and Morphogenesis lab, 75005 Paris, France. Electronic address: yohanns.bellaiche@curie.fr.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Mar 15; Vol. 5 (1), pp. 102851. Date of Electronic Publication: 2024 Feb 12.
DOI:	10.1016/j.xpro.2024.102851
Abstrakt:	Applying mechanical forces to tissues helps to understand morphogenesis and homeostasis. Additionally, recording the dynamics of living tissues under mechanical constraints is needed to explore tissue biomechanics. Here, we present a protocol to 3D-print a StretchCo device and use it to apply uniaxial mechanical stress on the Drosophila pupal dorsal thorax epithelium. We describe steps for 3D printing, polydimethylsiloxane (PDMS) strip cutting, and glue preparation. We detail procedures for PDMS strip mounting, tissue compaction, and live imaging upon force application. For additional details on the use and execution of this protocol, please refer to Cachoux et al. (2023) 1 from which the StretchCo machine has been derived. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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