Cannabidiol-loaded microparticles embedded in a porous hydrogel matrix for biomedical applications.

Autor: David C; Biopathological Research Group, Faculty of Dentistry (GIBFO), University of the Andes, Mérida, Venezuela. cldp58@gmail.com.; Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, Brazil. cldp58@gmail.com., de Souza JF; Laboratory of Technology and Development of Composites and Polymeric Materials-LaCoPol, Universidade Federal de Pelotas, Pelotas, Brazil., Silva AF; Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, Brazil., Grazioli G; Department of Dental Materials, Universidad de la República, Montevideo, Uruguay., Barboza AS; Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, Brazil., Lund RG; Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, Brazil., Fajardo AR; Laboratory of Technology and Development of Composites and Polymeric Materials-LaCoPol, Universidade Federal de Pelotas, Pelotas, Brazil., Moraes RR; Graduate Program in Dentistry, Universidade Federal de Pelotas, Pelotas, Brazil.
Jazyk: angličtina
Zdroj: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2024 Feb 14; Vol. 35 (1), pp. 14. Date of Electronic Publication: 2024 Feb 14.
DOI: 10.1007/s10856-023-06773-9
Abstrakt: In this study, poly (lactic-co-glycolic acid) (PLGA) microparticles loaded with cannabidiol (CBD) were synthesized (PLGA@CBD microparticles) and embedded up to 10 wt% in a chondroitin sulfate/polyvinyl alcohol hydrogel matrix. In vitro chemical, physical, and biological assays were carried out to validate the potential use of the modified hydrogels as biomaterials. The microparticles had spherical morphology and a narrow range of size distribution. CBD encapsulation efficiency was around 52%, loading was approximately 50%. Microparticle addition to the hydrogels caused minor changes in their morphology, FTIR and thermal analyses confirmed these changes. Swelling degree and total porosity were reduced in the presence of microparticles, but similar hydrophilic and degradation in phosphate buffer solution behaviors were observed by all hydrogels. Rupture force and maximum strain at rupture were higher in the modified hydrogels, whereas modulus of elasticity was similar across all materials. Viability of primary human dental pulp cells up to 21 days was generally not influenced by the addition of PLGA@CBD microparticles. The control hydrogel showed no antimicrobial activity against Staphylococcus aureus, whereas hydrogels with 5% and 10% PLGA@CBD microparticles showed inhibition zones. In conclusion, the PLGA@CBD microparticles were fabricated and successfully embedded in a hydrogel matrix. Despite the hydrophobic nature of CBD, the physicochemical and morphological properties were generally similar for the hydrogels with and without the CBD-loaded microparticles. The data reported in this study suggested that this original biomaterial loaded with CBD oil has characteristics that could enable it to be used as a scaffold for tissue/cellular regeneration.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: