Strategies for designing biocatalysts with new functions.

Autor:	Bell EL; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.; Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Anthony.green@manchester.ac.uk., Hutton AE; Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Anthony.green@manchester.ac.uk., Burke AJ; Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Anthony.green@manchester.ac.uk.; Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA., O'Connell A; School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland. elaine.oreilly@ucd.ie., Barry A; School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland. elaine.oreilly@ucd.ie., O'Reilly E; School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland. elaine.oreilly@ucd.ie., Green AP; Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Anthony.green@manchester.ac.uk.
Jazyk:	angličtina
Zdroj:	Chemical Society reviews [Chem Soc Rev] 2024 Mar 18; Vol. 53 (6), pp. 2851-2862. Date of Electronic Publication: 2024 Mar 18.
DOI:	10.1039/d3cs00972f
Abstrakt:	The engineering of natural enzymes has led to the availability of a broad range of biocatalysts that can be used for the sustainable manufacturing of a variety of chemicals and pharmaceuticals. However, for many important chemical transformations there are no known enzymes that can serve as starting templates for biocatalyst development. These limitations have fuelled efforts to build entirely new catalytic sites into proteins in order to generate enzymes with functions beyond those found in Nature. This bottom-up approach to enzyme development can also reveal new fundamental insights into the molecular origins of efficient protein catalysis. In this tutorial review, we will survey the different strategies that have been explored for designing new protein catalysts. These methods will be illustrated through key selected examples, which demonstrate how highly proficient and selective biocatalysts can be developed through experimental protein engineering and/or computational design. Given the rapid pace of development in the field, we are optimistic that designer enzymes will begin to play an increasingly prominent role as industrial biocatalysts in the coming years.
Databáze:	MEDLINE
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