Adult sex change leads to extensive forebrain reorganization in clownfish.

Autor:	Parker CG; Neuroscience Program, University of Illinois, Urbana-Champaign, Illinois, USA., Gruenhagen GW; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA., Hegarty BE; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA., Histed AR; Neuroscience Program, University of Illinois, Urbana-Champaign, Illinois, USA., Streelman JT; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA., Rhodes JS; Neuroscience Program, University of Illinois, Urbana-Champaign, Illinois, USA.; Department of Psychology, University of Illinois, Urbana-Champaign, Illinois, USA., Johnson ZV; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA.; Department of Psychiatry and Behavioral Sciences, Emory University, Atlanta, GA, USA.; Emory National Primate Research Center, Emory University, Atlanta, GA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jan 30. Date of Electronic Publication: 2024 Jan 30.
DOI:	10.1101/2024.01.29.577753
Abstrakt:	Sexual differentiation of the brain occurs in all major vertebrate lineages but is not well understood at a molecular and cellular level. Unlike most vertebrates, sex-changing fishes have the remarkable ability to change reproductive sex during adulthood in response to social stimuli, offering a unique opportunity to understand mechanisms by which the nervous system can initiate and coordinate sexual differentiation. This study explores sexual differentiation of the forebrain using single nucleus RNA-sequencing in the anemonefish Amphiprion ocellaris , producing the first cellular atlas of a sex-changing brain. We uncover extensive sex differences in cell type-specific gene expression, relative proportions of cells, baseline neuronal excitation, and predicted inter-neuronal communication. Additionally, we identify the cholecystokinin, galanin, and estrogen systems as central molecular axes of sexual differentiation. Supported by these findings, we propose a model of neurosexual differentiation in the conserved vertebrate social decision-making network spanning multiple subtypes of neurons and glia, including neuronal subpopulations within the preoptic area that are positioned to regulate gonadal differentiation. This work deepens our understanding of sexual differentiation in the vertebrate brain and defines a rich suite of molecular and cellular pathways that differentiate during adult sex change in anemonefish. Competing Interests: Competing Interest Statement: No competing interests.
Databáze:	MEDLINE
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