scMicrobe PTA: Near Complete Genomes from Single Bacterial Cells.

Autor:	Bowers RM; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Gonzalez-Pena V; Department of Pediatrics, Stanford University, Stanford, CA, USA., Wardhani K; Department of Pediatrics, Stanford University, Stanford, CA, USA., Goudeau D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Blow MJ; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Udwary D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Klein D; Department of Pediatrics, Stanford University, Stanford, CA, USA., Vill AC; Chan Zuckerberg Biohub, San Francisco, CA, USA., Brito IL; Chan Zuckerberg Biohub, San Francisco, CA, USA., Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Malmstrom R; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Gawad C; Department of Pediatrics, Stanford University, Stanford, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jan 31. Date of Electronic Publication: 2024 Jan 31.
DOI:	10.1101/2024.01.30.577819
Abstrakt:	Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters. Competing Interests: COMPETING INTERESTS CG and VGP are co-inventors on a patent related to this work. CG and VGP are equity holders of BioSkryb Genomics. CG is a co-founder and Board member of BioSkryb Genomics. The remaining authors declare no competing interests.
Databáze:	MEDLINE
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