| Autor: |
Sinha A, Park JM, Gulzar N, Pandya DN, Wadas TJ, Scott JK |
| Jazyk: |
angličtina |
| Zdroj: |
BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 02. Date of Electronic Publication: 2024 Feb 02. |
| DOI: |
10.1101/2024.01.31.578303 |
| Abstrakt: |
We report a functional pipeline for facile conversion of variable Fv domains, typically discovered in antibody discovery programs, into chimeric monoclonal antibodies (mAbs). Often, in initial screenings, a set of candidate mAbs is produced in small volumes and purified from supernatant for testing. Our pipeline also simplifies purification of mAbs by using an extended histidine tag (His-10) fused to the C-terminus of the light chain. Both the length of the His-10 and its location have been shown to affect the efficacy of mAb purification using an inexpensive nickel-based resin at neutral pH. Our antibody cloning and purification pipeline, when followed together with detection and affinity measurements, can be smoothly incorporated into an antibody discovery workflow. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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