Expansion of the split hygromycin toolkit for transgene insertion in Caenorhabditis elegans .
| Autor: | Moerdyk-Schauwecker MJ; Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States., Jahahn EK; Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States., Muñoz ZI; Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States., Robinson KJ; Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States., Phillips PC; Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Jan 29; Vol. 2024. Date of Electronic Publication: 2024 Jan 29 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001091 |
| Abstrakt: | Engineered sites for genetic transformation have simplified transgene insertion in Caenorhabditis elegans . These strategies include our split hygromycin system (Stevenson et al. 2020) which allows for integration-specific selection of transgenes. Here we have expanded the split hygromycin selection system to include two additional chromosomal locations, both of which are permissive for germline expression, as well as engineered landing pads in three additional natural isolates. Corresponding guide and empty repair template plasmids are also available for each of these sites. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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