CdsA, a CDP-diacylglycerol synthase involved in phospholipid and glycolipid MPIase biosynthesis, possesses multiple initiation codons.

Autor: Hikage R; Department of Applied Biological Chemistry, Faculty of Agriculture, Iwate University, Morioka, Japan., Sekiya Y; Department of Applied Biological Chemistry, Faculty of Agriculture, Iwate University, Morioka, Japan., Sawasato K; Department of Applied Biological Chemistry, Faculty of Agriculture, Iwate University, Morioka, Japan., Nishiyama KI; Department of Applied Biological Chemistry, Faculty of Agriculture, Iwate University, Morioka, Japan.
Jazyk: angličtina
Zdroj: Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2024 Apr; Vol. 29 (4), pp. 347-355. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1111/gtc.13104
Abstrakt: CdsA is a CDP-diacylglycerol synthase essential for phospholipid and glycolipid MPIase biosynthesis, and therefore for growth. The initiation codon of CdsA has been assigned as "TTG," while methionine at the 37th codon was reported to be an initiation codon in the original report. Since a vector containing the open reading frame starting with "TTG" under a controllable promoter complemented the cdsA knockout, "TTG" could function as an initiation codon. However, no evidence supporting that this "TTG" is the sole initiation codon has been reported. We determined the initiation codon by examining the ability of mutants around the N-terminal region to complement cdsA mutants. Even if the "TTG" was substituted with a stop codon, the clear complementation was observed. Moreover, the clones with multiple mutations of stop codons complemented the cdsA mutant up to the 37th codon, indicating that cdsA possesses multiple codons that can function as initiation codons. We constructed an experimental system in which the chromosomal expression of cdsA can be analyzed. By means of this system, we found that the cdsA mutant with substitution of "TTG" with a stop codon is fully functional. Thus, we concluded that CdsA contains multiple initiation codons.
(© 2024 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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