Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovis.

Autor: Démoulins T; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland. thomas.demoulins@unibe.ch., Yimthin T; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland., Lindtke D; Animal GenoPhenomics Group, Agroscope, 1725, Posieux, Switzerland., Eggerschwiler L; Research Contracts Animals Group, Agroscope, 1725, Posieux, Switzerland., Siegenthaler R; Research Contracts Animals Group, Agroscope, 1725, Posieux, Switzerland., Labroussaa F; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.; Multidisciplinary Center for Infectious Diseases (MCID), University of Bern, 3001, Bern, Switzerland., Jores J; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001, Bern, Switzerland.; Multidisciplinary Center for Infectious Diseases (MCID), University of Bern, 3001, Bern, Switzerland.
Jazyk: angličtina
Zdroj: Veterinary research [Vet Res] 2024 Feb 13; Vol. 55 (1), pp. 18. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1186/s13567-024-01272-3
Abstrakt: Although cattle are the mammalian species with most global biomass associated with a huge impact on our planet, their immune system remains poorly understood. Notably, the bovine immune system has peculiarities such as an overrepresentation of γδ T cells that requires particular attention, specifically in an infectious context. In line of 3R principles, we developed an ex vivo platform to dissect host-pathogen interactions. The experimental design was based on two independent complementary readouts: firstly, a novel 12-14 color multiparameter flow cytometry assay measuring maturation (modulation of cell surface marker expression) and activation (intracellular cytokine detection) of monocytes, conventional and plasmacytoid dendritic cells, natural killer cells, γδ T cells, B and T cells; secondly, a multiplex immunoassay monitoring bovine chemokine and cytokine secretion levels. The experiments were conducted on fresh primary bovine blood cells exposed to Mycoplasmopsis bovis (M. bovis), a major bovine respiratory pathogen. Besides reaffirming the tight cooperation of the different primary blood cells, we also identified novel key players such as strong IFN-γ secreting NK cells, whose role was so far largely overlooked. Additionally, we compared the host-pathogen interactions at different temperatures, including commonly used 37 °C, ruminant body temperature (38-38.5 °C) and fever (≥ 39.5 °C). Strikingly, working under ruminant physiological temperature influenced the capacity of most immune cell subsets to respond to M. bovis compared to 37 °C. Under fever-like temperature conditions the immune response was impaired compared to physiological temperature. Our experimental approach, phenotypically delineating the bovine immune system provided a thorough vision of the immune response towards M. bovis and the influence of temperature towards that immune response.
(© 2024. The Author(s).)
Databáze: MEDLINE
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