Marine cyanobacterial biomass is an efficient feedstock for fungal bioprocesses.

Autor: Gupta JK; Systems Biology for Biofuel Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), ICGEB Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.; Zero Cow Factory, Surat, India., Jain KK; DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi, 110067, India.; The Live Green Co., Bangalore, India., Kaushal M; Systems Biology for Biofuel Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), ICGEB Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.; Perfect Day India Pvt. Ltd., Bangalore, India., Upton DJ; Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Joshi M; DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi, 110067, India.; Biocon Limited, Bangalore, India., Pachauri P; Systems Biology for Biofuel Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), ICGEB Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India., Wood AJ; Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK.; Department of Mathematics, University of York, York, YO10 5DD, UK., Yazdani SS; DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi, 110067, India.; Microbial Engineering Group, ICGEB, New Delhi, 110067, India., Srivastava S; Systems Biology for Biofuel Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), ICGEB Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India. shireesh@icgeb.res.in.; DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi, 110067, India. shireesh@icgeb.res.in.
Jazyk: angličtina
Zdroj: Biotechnology for biofuels and bioproducts [Biotechnol Biofuels Bioprod] 2024 Feb 13; Vol. 17 (1), pp. 23. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1186/s13068-024-02469-6
Abstrakt: Background: Marine cyanobacteria offer many sustainability advantages, such as the ability to fix atmospheric CO 2 , very fast growth and no dependence on freshwater for culture. Cyanobacterial biomass is a rich source of sugars and proteins, two essential nutrients for culturing any heterotroph. However, no previous study has evaluated their application as a feedstock for fungal bioprocesses.
Results: In this work, we cultured the marine cyanobacterium Synechococcus sp. PCC 7002 in a 3-L externally illuminated bioreactor with working volume of 2 L with a biomass productivity of ~ 0.8 g L -1  day -1 . Hydrolysis of the biomass with acids released proteins and hydrolyzed glycogen while hydrolysis of the biomass with base released only proteins but did not hydrolyze glycogen. Among the different acids tested, treatment with HNO 3 led to the highest release of proteins and glucose. Cyanobacterial biomass hydrolysate (CBH) prepared in HNO 3 was used as a medium to produce cellulase enzyme by the Penicillium funiculosum OAO3 strain while CBH prepared in HCl and treated with charcoal was used as a medium for citric acid by Aspergillus tubingensis. Approximately 50% higher titers of both products were obtained compared to traditional media.
Conclusions: These results show that the hydrolysate of marine cyanobacteria is an effective source of nutrients/proteins for fungal bioprocesses.
(© 2024. The Author(s).)
Databáze: MEDLINE
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