A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence.

Autor: Chen PK; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan., Lee YT; Department of Emergency Medicine, Taipei Veteran General Hospital, Taipei, Taiwan; School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan., Liu CY; Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan., Thuy TTD; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan., Anh K; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan., Wu JJ; Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan., Liao CH; Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan., Huang YT; Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan., Chen YC; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Kao CY; Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan; Health Innovation Center, National Yang Ming Chiao Tung University, Taiwan; Microbiota Research Center, National Yang Ming Chiao Tung University, Taiwan. Electronic address: kaocy@nycu.edu.tw.
Jazyk: angličtina
Zdroj: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [J Microbiol Immunol Infect] 2024 Apr; Vol. 57 (2), pp. 288-299. Date of Electronic Publication: 2024 Feb 08.
DOI: 10.1016/j.jmii.2024.01.008
Abstrakt: Background: This study aimed to characterize carbapenem-nonsusceptible Acinetobacter (CNSA) isolated from patients with bacteremia from 1997 to 2015.
Methods: A total of 173 CNSA (12.3%) was recovered from 1403 Acinetobacter isolates. The presence of selected β-lactamase genes in CNSA was determined by PCR amplification. The conjugation test was used to determine the transferability of metallo-β-lactamase (MBL)-carrying plasmids. Whole genome sequencing in combination with phenotypic assays was carried out to characterize MBL-plasmids.
Results: In general, a trend of increasing numbers of CNSA was observed. Among the 173 CNSA, A. baumannii (54.9%) was the most common species, followed by A. nosocomialis (23.1%) and A. soli (12.1%). A total of 49 (28.3%) CNSA were extensively drug-resistant, and all were A. baumannii. The most common class D carbapenemase gene in 173 CNSA was bla OXA-24-like (32.4%), followed by ISAba1-bla OXA-51-like (20.8%), ISAba1-bla OXA-23 (20.2%), and IS1006/IS1008-bla OXA-58 (11.6%). MBL genes, bla VIM-11, bla IMP-1 , and bla IMP-19 were detected in 9 (5.2%), 20 (11.6%), and 1 (0.6%) CNSA isolates, respectively. Transfer of MBL genes to AB218 and AN254 recipient cells was successful for 7 and 6 of the 30 MBL-plasmids, respectively. The seven AB218-derived transconjugants carrying MBL-plasmids produced less biofilm but showed higher virulence to larvae than recipient AB218.
Conclusions: Our 19-year longitudinal study revealed a stable increase in CNSA during 2005-2015. bla OXA-24-like , ISAba1-bla OXA-51-like , and ISAba1-bla OXA-23 were the major determinants of Acinetobacter carbapenem resistance. MBL-carrying plasmids contribute not only to the carbapenem resistance but also to A. baumannii virulence.
Competing Interests: Declaration of competing interest The authors report that there are no competing interests to declare.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: