Revisiting rabbit models for keratoconus: A long-term study on collagenase-induced disease progression.

Autor: Shankar S; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India., Thacker M; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India., Sahoo A; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India; UT-ORNL Graduate School of Genome Science and Technology, Bredesen Centre, University of Tennessee at Knoxville, Knoxville, USA., Aindla AR; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India., Padala KR; CSIR-Centre for Cellular and Molecular Biology, ANNEXE II, Medical Biotechnology Complex, Uppal Road, Hyderabad, Telangana, India., Jaffet J; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India., Bokara KK; CSIR-Centre for Cellular and Molecular Biology, ANNEXE II, Medical Biotechnology Complex, Uppal Road, Hyderabad, Telangana, India. Electronic address: bokarakiran@ccmb.res.in., Basu S; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India; Shantilal Shanghvi Cornea Institute, L.V. Prasad Eye Institute, Hyderabad, Telangana, India. Electronic address: sayanbasu@lvpei.org., Singh V; Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L.V. Prasad Eye Institute, Hyderabad, Telangana, India. Electronic address: viveksingh@lvpei.org.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2024 Apr; Vol. 241, pp. 109811. Date of Electronic Publication: 2024 Feb 11.
DOI: 10.1016/j.exer.2024.109811
Abstrakt: Keratoconus (KC) is a degenerative disorder resulting from the degradation of the stromal collagen fibril network in the cornea, leading to its thinning and conical deformation. Various studies have established animal models of KC by using the collagenase type II enzyme to gain a better understanding of the pathogenesis, however, long-term monitoring or follow-up of the models have not been reported so far. This study evaluates the long-term stability of collagenase type II-induced KC in a rabbit model. Six New Zealand rabbits were divided into 4 study groups with 3 eyes per group. The groups were control (group 1), 0.5% proparacaine + 5 min collagenase treatment on day 0 and day 30 (group 2), 0.5% proparacaine + 10 min collagenase treatment on day 0 (group 3) and, mechanical debridement + 2 min collagenase treatment on day 0 (group 4). Inflammation was observed in group 4 till week 10. Significant decrease in the central corneal thickness was observed in group 3 by week 4 (p < 0.001) however, the thickness was regained in the subsequent follow-ups in all the groups. Keratography results showed no changes in Km values but an increased astigmatic power in all groups. Scanning electron microscopy images revealed thinner collagen fibrils arranged in a mesh-like pattern above the uniform layer of the collagen lamellae in the central part of the treated corneas. Similarly, histological staining revealed loosely packed stromal fibrils in the anterior portion of the cornea which corroborates with the immunofluorescent staining results. This study revealed the remodeling of the corneal structure by eight weeks of collagenase treatment. Consequently, the possibility of creating a rabbit keratoconus model induced by collagenase may warrant further consideration.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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