Evaluation of chemical disposition in skin by stimulated Raman scattering microscopy.

Autor: Zarmpi P; University of Bath, Department of Life Sciences, Claverton Down, Bath BA2 7AY, UK., Tsikritsis D; National Physical Laboratory, Teddington TW11 0LW, UK., Vorng JL; National Physical Laboratory, Teddington TW11 0LW, UK., Belsey NA; National Physical Laboratory, Teddington TW11 0LW, UK; University of Surrey, School of Chemistry & Chemical Engineering, Guildford GU2 7XH, UK., Bunge AL; Colorado School of Mines, Department of Chemical & Biological Engineering, Golden, CO 80401, USA., Woodman TJ; University of Bath, Department of Life Sciences, Claverton Down, Bath BA2 7AY, UK., Delgado-Charro MB; University of Bath, Department of Life Sciences, Claverton Down, Bath BA2 7AY, UK., Guy RH; University of Bath, Department of Life Sciences, Claverton Down, Bath BA2 7AY, UK. Electronic address: r.h.guy@bath.ac.uk.
Jazyk: angličtina
Zdroj: Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2024 Apr; Vol. 368, pp. 797-807. Date of Electronic Publication: 2024 Mar 22.
DOI: 10.1016/j.jconrel.2024.02.011
Abstrakt: Tracking drug disposition in the skin in a non-destructive and at least semi-quantitative fashion is a relevant objective for the assessment of local (cutaneous) bioavailability. Confocal Raman spectroscopy has been shown potentially useful in this regard and, importantly, recent advances have enabled the presence of applied chemicals in the viable epidermis below the stratum corneum (SC) to be determined without ambiguity and having addressed the challenges of (a) background signals from endogenous species and noise and (b) signal attenuation due to absorption and scattering. This study aimed to confirm these observations using a different vibrational spectroscopy approach - specifically, stimulated Raman scattering (SRS) microscopy - and the more conventional in vitro skin penetration test (IVPT). SRS is a nonlinear optical imaging technique which enables more precise location of the skin surface and enhanced skin depth resolution relative to confocal Raman microscopy. The method can also probe larger areas of the sample under investigation and identify the localization of the permeating chemical in specific structural components of the skin. Here, SRS was shown capable of tracking the uptake and distribution of 4-cyanophenol (CP), the same model compound used in the recent confocal Raman investigation, at depths beyond the SC following skin treatment with different vehicles and for different times. The SRS results correlated well with those from the confocal Raman experiments, and both were consistent with independent IVPT measurements. Acquired images clearly delineated CP preference for the intercellular lipid layers of the SC relative to the corneocytes. The stage is now set to apply these and other correlative techniques to examine commercial drug products.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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