Circulating exosome-mediated AMPKα-SIRT1 pathway regulates lipid metabolism disorders in calf hepatocytes.

Autor: Zhang D; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Ding H; Research Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, Anhui Province 230031, China., Liu C; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Huang Y; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Tai W; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Feng S; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Wang X; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China., Zhao C; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China. Electronic address: chang_zhao@ahau.edu.cn., Li Y; College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui Province 230036, China. Electronic address: lydhy2014@ahau.edu.cn.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2024 Mar; Vol. 169, pp. 105177. Date of Electronic Publication: 2024 Feb 06.
DOI: 10.1016/j.rvsc.2024.105177
Abstrakt: Subclinical ketosis (SCK) in dairy cows is often misdiagnosed because it lacks clinical signs and detection indicators. However, it is highly prevalent and may transform into clinical ketosis if not treated promptly. Due to the negative energy balance, a large amount of fat is mobilized, producing NEFA that exceeds the upper limit of liver processing, which in turn leads to the disturbance of liver lipid metabolism. The silent information regulator 1 (SIRT1) is closely related to hepatic lipid metabolism disorders. Exosomes as signal transmitters, also play a role in the circulatory system. We hypothesize that the circulating exosome-mediated adenosine 5'-monophosphate (AMP)-activated protein kinase alpha (AMPKα)-SIRT1 pathway regulates lipid metabolism disorders in SCK cows. We extracted the exosomes required for the experiment from the peripheral circulating blood of non-ketotic (NK) and SCK cows. We investigated the effect of circulating exosomes on the expression levels of mRNA and protein of the AMPKα-SIRT1 pathway in non-esterified fatty acid (NEFA)-induced dairy cow primary hepatocytes using in vitro cell experiments. The results showed that circulating exosomes increased the expression levels of Lipolysis-related genes and proteins (AMPKα, SIRT1, and PGC-1α) in hepatocytes treated with 1.2 mM NEFA, and inhibited the expression of lipid synthesis-related genes and protein (SREBP-1C). The regulation of exosomes on lipid metabolism disorders caused by 1.2 mM NEFA treatment showed the same trend as for SIRT1-overexpressing adenovirus. The added exosomes could regulate NEFA-induced lipid metabolism in hepatocytes by mediating the AMPKα-SIRT1 pathway, consistent with the effect of transfected SIRT1 adenovirus.
Competing Interests: Declaration of competing interest The authors declare no potential or real conflicts of interest.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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