YfmR is a translation factor that prevents ribosome stalling and cell death in the absence of EF-P.

Autor:	Hong HR; Department of Microbiology, Cornell University, Ithaca, NY 14853., Prince CR; Department of Microbiology, Cornell University, Ithaca, NY 14853., Tetreault DD; Department of Microbiology, Cornell University, Ithaca, NY 14853., Wu L; Department of Microbiology, Cornell University, Ithaca, NY 14853., Feaga HA; Department of Microbiology, Cornell University, Ithaca, NY 14853.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Feb 20; Vol. 121 (8), pp. e2314437121. Date of Electronic Publication: 2024 Feb 13.
DOI:	10.1073/pnas.2314437121
Abstrakt:	Protein synthesis is performed by the ribosome and a host of highly conserved elongation factors. Elongation factor P (EF-P) prevents ribosome stalling at difficult-to-translate sequences, such as polyproline tracts. In bacteria, phenotypes associated with efp deletion range from modest to lethal, suggesting that some species encode an additional translation factor that has similar function to EF-P. Here we identify YfmR as a translation factor that is essential in the absence of EF-P in Bacillus subtilis . YfmR is an ABCF ATPase that is closely related to both Uup and EttA, ABCFs that bind the ribosomal E-site and are conserved in more than 50% of bacterial genomes. We show that YfmR associates with actively translating ribosomes and that depleting YfmR from Δ efp cells causes severe ribosome stalling at a polyproline tract in vivo. YfmR depletion from Δ efp cells was lethal and caused reduced levels of actively translating ribosomes. Our results therefore identify YfmR as an important translation factor that is essential in B. subtilis in the absence of EF-P. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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