New Fluorescent Dye for the Detection of Zn 2+ in Living Cells and Fixed Sections of the Rat Pancreas.

Autor: Krinochkin A; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; I. Ya. Postovskiy Institute of Organic Synthesis, Ural Branch of the RAS, 22 S. Kovalevskoy Street, 620219, Yekaterinburg, Russia., Valieva M; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; I. Ya. Postovskiy Institute of Organic Synthesis, Ural Branch of the RAS, 22 S. Kovalevskoy Street, 620219, Yekaterinburg, Russia., Starnovskaya E; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia., Slovesnova N; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; Urals State Medical University, 3 Repina Street, 620028, Yekaterinburg, Russia., Minin A; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; M.N. Miheev Institute of Metal Physics, Ural Branch of the RAS, 18 S. Kovalevskoy Street, 620108, Yekaterinburg, Russia., Belousova A; Institute of Immunology and Physiology, Ural Branch of the RAS, 106 Pervomaiskaya Street, 620049, Yekaterinburg, Russia., Sadieva L; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia. l.k.sadieva@urfu.ru., Taniya O; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia., Khasanov A; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia., Novikov A; Institute of Chemistry, Saint Petersburg State University, 7/9 Universitetskaya Nab., 199034, Saint Petersburg, Russia., Bruskov V; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia., Vatolina S; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia., Kopchuk D; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; I. Ya. Postovskiy Institute of Organic Synthesis, Ural Branch of the RAS, 22 S. Kovalevskoy Street, 620219, Yekaterinburg, Russia., Slepukhin P; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; I. Ya. Postovskiy Institute of Organic Synthesis, Ural Branch of the RAS, 22 S. Kovalevskoy Street, 620219, Yekaterinburg, Russia., Sharutin V; Department of Chemistry, Institute of Natural Sciences, South Ural State University (National Research University), Lenin Avenue 76, 454080, Chelyabinsk, Russia., Zyryanov G; Ural Federal University, 19 Mira Street, 620002, Yekaterinburg, Russia.; I. Ya. Postovskiy Institute of Organic Synthesis, Ural Branch of the RAS, 22 S. Kovalevskoy Street, 620219, Yekaterinburg, Russia.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2024 Feb 13. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1007/s10895-024-03603-1
Abstrakt: We report the synthesis and characterization of a new 4-methoxyphenyl-2,2'-bipyridine-based ligand, such as 12, bearing dipicolylaminomethyl core as a receptor unit, as a probe for the fluorescence "turn-on" detection of Zn 2+ . Thus, in the presence of Zn 2+ the probe 12 exhibited a fluorescence enhancement with a Stokes shift of ~ 180 nm and photoluminescence quantum yields value of ~ 1.0. In addition, 12 exhibited higher binding constant for Zn 2+ (~ 2 × 10 5  M -1 ) with the LOD reaching the nanomolar level (~ 0.1 × 10 -9  M) compare to the previously reported probe 1. The stoichiometry and structure of the [Zn(12)] 2+ and [Zn(1)] 2+ complexes were supported by XRD analysis, DFT calculations and 1 H NMR experiments. It was postulated that, as a result of binding of Zn 2+ , the sample exhibited a bright "on" state via the PET-ICT processes. Molecular docking studies and confocal fluorescence microscopy experiments demonstrated that the probe 12 could be used for the fluorescence detection of Zn 2+ not only in artificially enriched with zinc salts live cells, but also in fixed tissues with cations are in a bound state. The high binding constant of compound 12 to Zn 2+ cation allows it to be used for the accurate localization of pancreatic beta cells (islets of Langerhans).
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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