Cyanidin prevents cardiomyocyte apoptosis in mice after myocardial infarction.

Autor: Gang D; School of Medicine, South China University of Technology, Guangzhou, 510006, People's Republic of China.; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China., Qing O; School of Medicine, South China University of Technology, Guangzhou, 510006, People's Republic of China.; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Yang Y; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Masood M; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Center for Discovery and Innovation, Hackensack University Medicial Center, Nutley, NJ, USA., Wang YH; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Linhui J; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Haotao S; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Li G; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China., Liu C; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China. liuchi1985@163.com.; Department of Nephrology, Sichuan Academy of Medical Science and Sichuan, Provincial People's Hospital, Sichuan Renal Disease Clinical Research Center, University of Electronic Science and Technology of China, Chengdu, People's Republic of China. liuchi1985@163.com., Nasser MI; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China. moussa@gdph.org.cn.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China. moussa@gdph.org.cn., Zhu P; School of Medicine, South China University of Technology, Guangzhou, 510006, People's Republic of China. tanganqier@163.com.; Department of Cardiac Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangdong Cardiovascular Institute, Guangzhou, Guangdong, 510100, People's Republic of China. tanganqier@163.com.; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China. tanganqier@163.com.; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China. tanganqier@163.com.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2024 Aug; Vol. 397 (8), pp. 5883-5898. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1007/s00210-024-02975-2
Abstrakt: Myocardial infarction is a worldwide disease with high morbidity and mortality and a major cause of chronic heart failure, seriously affecting patients' quality of life. Natural medicine has been used to cure or prevent cardiovascular disease for decades. As a natural flavonoid, anthocyanidin has been used to treat many diseases due to its antioxidative, anti-inflammatory, and other properties. A mouse model (C57BL/6) weighing 30-40 g was utilized to induce myocardial infarction by ligating the left anterior descending coronary artery. Cyanidin (30 mg/kg) was administered orally to mice for four weeks. A variety of assessments were used to evaluate cardiac function. The gene expression was measured using RNAseq and Western blot. Histological changes in myocardial tissue were assessed using staining techniques, including Masson, Hematoxylin Eosin (HE), and transmission electron microscopy. Tunnel staining was implemented as a method to detect cellular apoptosis. For the quantification of B-type natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) in the serum, an enzyme-linked immunosorbent assay (ELISA) was employed. Furthermore, autodock simulation was executed in order to assess the interaction between cyanidin and a subset of genes. Cyanidin treatment inhibited myocardial cell apoptosis, improved cardiac function, and reduced serum concentrations of BNP and atrial natriuretic peptide ANP, as well as mitigated histological cardiac tissue damage. Cyanidin also inhibited the activity of matrix metalloproteinases (MMP2/9) and Fibronectin 1 (Fn1). Cyanidin improves heart function and reduces myocardial damage in mice after MI. Furthermore, cyanidin can prevent cardiomyocyte apoptosis. These effects are most likely caused by suppression of MMP9/2 and control of the Akt signaling pathway, suggesting an appropriate therapeutic target.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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