| Autor: |
Şancı E; Center for Drug Development and Pharmacokinetic Applications, Ege University, Bornova Izmir, Turkey., Köksal Karayıldırım Ç; Department of Biology, Ege University, Bornova Izmir, Turkey., Dağdeviren M; Department of Biology, Ege University, Bornova Izmir, Turkey., Yiğittürk G; Department of Histology and Embryology, Muğla Sıtkı Koçman University, Mugla, Turkey., Buhur A; Department of Histology and Embryology, Ege University, Bornova Izmir, Turkey., Erbaş O; Department of Physiology, Demiroğlu Bilim University, Istanbul, Turkey., Yavaşoğlu A; Department of Histology and Embryology, Ege University, Bornova Izmir, Turkey., Karabay Yavaşoğlu NÜ; Center for Drug Development and Pharmacokinetic Applications, Ege University, Bornova Izmir, Turkey.; Department of Biology, Ege University, Bornova Izmir, Turkey. |
| Jazyk: |
angličtina |
| Zdroj: |
Drug and chemical toxicology [Drug Chem Toxicol] 2024 Nov; Vol. 47 (6), pp. 933-948. Date of Electronic Publication: 2024 Feb 13. |
| DOI: |
10.1080/01480545.2024.2315150 |
| Abstrakt: |
Streptozotocin (STZ) is used as a diabetes-inducing agent in experimental animal studies. However, it is known that STZ-induced diabetic animals show significant increases in oxidative stress parameters and neurodegeneration besides their blood glucose level. In this study, the acute and subacute toxic effects of STZ on the liver, sciatic nerve, and brain tissues were investigated in vivo rat model. Sprague-Dawley rats were divided into two groups; while 50 mg/kg STZ was administered ip to the STZ group, only saline was administered to the control group. After STZ administration, three units (100 U/mL) of subcutaneous insulin glargine were applied daily to prevent the formation of diabetes. At 24 h, 1,2, and 4 weeks after applications, rats from each group were sacrificed and tissues were removed under anesthesia. At the end of the study, compared to the control, a significant decrease in SOD and GST activity and an increase in lipid peroxidation were detected in the liver and sciatic tissues of rats in the STZ-treated group in the first 24h. Considering the TUNEL, NFκB, and NOS2 expressions, it was noted that while the effects of STZ on the liver were observed in the acute stage (24h), it had subacute effects on the brain. When apoptosis-related gene expression ( Bcl-2, Bax, CASP3, CASP8, CASP9, TNF-α ) and immunohistochemistry were evaluated, the apoptotic effect of STZ was observed mostly in sciatic nerve tissues. Within the scope of the study, it was revealed that STZ did not only show selective toxicity to pancreatic β cells but also very toxic to other tissues and organs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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