Understanding bovine embryo elongation: a transcriptomic study of trophoblastic vesicles.

Autor:	Degrelle SA; Université Paris-Saclay, Université Versailles Saint-Quentin en Yvelines, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Biologie de la Reproduction, Environnement, Epigénétique et Développment, Jouy en Josas, France.; Inovarion, Paris, France., Liu F; Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China., Laloe D; Université Paris Saclay, INRAE, AgroParisTech, GABI, Domaine de Vilvert, Jouy en Josas, France., Richard C; Université Paris-Saclay, Université Versailles Saint-Quentin en Yvelines, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Biologie de la Reproduction, Environnement, Epigénétique et Développment, Jouy en Josas, France., Le Bourhis D; ELIANCE, Nouzilly, France., Rossignol MN; Université Paris Saclay, INRAE, AgroParisTech, GABI, Domaine de Vilvert, Jouy en Josas, France., Hue I; Université Paris-Saclay, Université Versailles Saint-Quentin en Yvelines, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Biologie de la Reproduction, Environnement, Epigénétique et Développment, Jouy en Josas, France.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2024 Jan 29; Vol. 15, pp. 1331098. Date of Electronic Publication: 2024 Jan 29 (Print Publication: 2024).
DOI:	10.3389/fphys.2024.1331098
Abstrakt:	Background: During the process of elongation, the embryo increases in size within the uterus, while the extra-embryonic tissues (EETs) develop and differentiate in preparation for implantation. As it grows, the ovoid embryo transforms into a tubular form first and then a filamentous form. This process is directed by numerous genes and pathways, the expression of which may be altered in the case of developmental irregularities such as when the conceptus is shorter than expected or when the embryo develops after splitting. In bovines, efforts to understand the molecular basis of elongation have employed trophoblastic vesicles (TVs)-short tubular EET pieces that lack an embryo-which also elongate in vivo . To date, however, we lack molecular analyses of TVs at the ovoid or filamentous stages that might shed light on the expression changes involved. Methods: Following in vivo development, we collected bovine conceptuses from the ovoid (D12) to filamentous stages (D18), sectioned them into small pieces with or without their embryonic disc (ED), and then, transferred them to a receptive bovine uterus to assess their elongation abilities. We also grew spherical blastocysts in vitro up to D8 and subjected them to the same treatment. Then, we assessed the differences in gene expression between different samples and fully elongating controls at different stages of elongation using a bovine array (10 K) and an extended qPCR array comprising 224 genes across 24 pathways. Results: In vivo , TVs elongated more or less depending on the stage at which they had been created and the time spent in utero . Their daily elongation rates differed from control EET, with the rates of TVs sometimes resembling those of earlier-stage EET. Overall, the molecular signatures of TVs followed a similar developmental trajectory as intact EET from D12-D18. However, within each stage, TVs and intact EET displayed distinct expression dynamics, some of which were shared with other short epithelial models. Conclusion: Differences between TVs and EET likely result from multiple factors, including a reduction in the length and signaling capabilities of TVs, delayed elongation from inadequate uterine signals, and modified crosstalk between the conceptus and the uterus. These findings confirm that close coordination between uterine, embryonic, and extra-embryonic tissues is required to orchestrate proper elongation and, based on the partial differentiation observed, raise questions about the presence/absence of certain developmental cues or even their asynchronies. Competing Interests: The authors SAD and DL were employed by the company Inovarion and ELIANCE, respectively. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Degrelle, Liu, Laloe, Richard, Le Bourhis, Rossignol and Hue.)
Databáze:	MEDLINE
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