A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.

Autor: Dunuweera AN; Department of Botany, University of Jaffna, Jaffna 40000, Sri Lanka., Dunuweera SP; Department of Chemistry, University of Peradeniya, Peradeniya 20400, Sri Lanka., Ranganathan K; Department of Botany, University of Jaffna, Jaffna 40000, Sri Lanka.
Jazyk: angličtina
Zdroj: Biochemistry research international [Biochem Res Int] 2024 Feb 05; Vol. 2024, pp. 8273237. Date of Electronic Publication: 2024 Feb 05 (Print Publication: 2024).
DOI: 10.1155/2024/8273237
Abstrakt: Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light. Although there are many bioluminescent systems, few have been identified. D-luciferin-dependent systems, coelenterazine-dependent systems, Cypridina luciferin-based systems, tetrapyrrole-based luciferins, bacterial bioluminescent systems, and fungal bioluminescent systems are natural bioluminescent systems. Since different bioluminescence systems, such as various combinations of luciferin-luciferase pair reactions, have different light emission wavelengths, they benefit industrial applications such as drug discovery, protein-protein interactions, in vivo imaging in small animals, and controlling neurons. Due to the expression of luciferase and easy permeation of luciferin into most cells and tissues, bioluminescence assays are applied nowadays with modern technologies in most cell and tissue types. It is a versatile technique in a variety of biomedical research. Furthermore, there are some investigated blue-sky research projects, such as bioluminescent plants and lamps. This review article is mainly based on the theory of diverse bioluminescence systems and their past, present, and future applications.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2024 Asiri N. Dunuweera et al.)
Databáze: MEDLINE
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