Genomic attributes of airway commensal bacteria and mucosa.

Autor: Cuthbertson L; National Heart and Lung Institute, Imperial College London, London, UK., Löber U; Max Delbrück Center for Molecular Medicine (MDC), 13125, Berlin, Germany.; Experimental and Clinical Research Center, A Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Lindenberger Weg 80, 13125, Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), Partner Site, 10785, Berlin, Germany.; Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Berlin, Germany., Ish-Horowicz JS; National Heart and Lung Institute, Imperial College London, London, UK.; Department of Mathematics, Imperial College London, London, UK., McBrien CN; National Heart and Lung Institute, Imperial College London, London, UK., Churchward C; National Heart and Lung Institute, Imperial College London, London, UK., Parker JC; National Heart and Lung Institute, Imperial College London, London, UK., Olanipekun MT; National Heart and Lung Institute, Imperial College London, London, UK., Burke C; Department of Respiratory Medicine, Connolly Hospital, Dublin, Ireland., McGowan A; Department of Respiratory Medicine, Connolly Hospital, Dublin, Ireland., Davies GA; Population Data Science and Health Data Research UK BREATHE Hub, Swansea University Medical School, Swansea University, Swansea, UK.; College of Medicine, Institute of Life Science, Swansea University, Swansea, UK., Lewis KE; College of Medicine, Institute of Life Science, Swansea University, Swansea, UK.; Respiratory Medicine, Hywel Dda University Health Board, Llanelli, UK., Hopkin JM; College of Medicine, Institute of Life Science, Swansea University, Swansea, UK., Chung KF; National Heart and Lung Institute, Imperial College London, London, UK., O'Carroll O; Department of Respiratory Medicine, Connolly Hospital, Dublin, Ireland., Faul J; Department of Respiratory Medicine, Connolly Hospital, Dublin, Ireland., Creaser-Thomas J; College of Medicine, Institute of Life Science, Swansea University, Swansea, UK., Andrews M; Respiratory Medicine, Hywel Dda University Health Board, Llanelli, UK., Ghosal R; Respiratory Medicine, Hywel Dda University Health Board, Llanelli, UK., Piatek S; National Heart and Lung Institute, Imperial College London, London, UK., Willis-Owen SAG; National Heart and Lung Institute, Imperial College London, London, UK., Bartolomaeus TUP; Max Delbrück Center for Molecular Medicine (MDC), 13125, Berlin, Germany.; Experimental and Clinical Research Center, A Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Lindenberger Weg 80, 13125, Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), Partner Site, 10785, Berlin, Germany.; Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Berlin, Germany., Birkner T; Max Delbrück Center for Molecular Medicine (MDC), 13125, Berlin, Germany.; Experimental and Clinical Research Center, A Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Lindenberger Weg 80, 13125, Berlin, Germany.; Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Berlin, Germany., Dwyer S; National Heart and Lung Institute, Imperial College London, London, UK., Kumar N; Host-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Turek EM; National Heart and Lung Institute, Imperial College London, London, UK., William Musk A; School of Population and Global Health, The University of Western Australia, Perth, WA, Australia.; Busselton Population Medical Research Institute, Sir Charles Gairdner Hospital, Perth, WA, Australia.; Department of Respiratory Medicine Sir Charles Gairdner Hospital, Perth, WA, Australia., Hui J; School of Population and Global Health, The University of Western Australia, Perth, WA, Australia.; Busselton Population Medical Research Institute, Sir Charles Gairdner Hospital, Perth, WA, Australia., Hunter M; School of Population and Global Health, The University of Western Australia, Perth, WA, Australia.; Busselton Population Medical Research Institute, Sir Charles Gairdner Hospital, Perth, WA, Australia., James A; School of Population and Global Health, The University of Western Australia, Perth, WA, Australia.; Department of Respiratory Medicine Sir Charles Gairdner Hospital, Perth, WA, Australia.; Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Perth, WA, Australia., Dumas ME; National Heart and Lung Institute, Imperial College London, London, UK.; Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; U1283 INSERM / UMR8199 CNRS, Institut Pasteur de Lille, Lille University Hospital, European Genomic Institute for Diabetes, University of Lille, Lille, France.; McGill Genome Centre, McGill University, Montréal, QC, Canada., Filippi S; Department of Mathematics, Imperial College London, London, UK., Cox MJ; University of Birmingham College of Medical and Dental Sciences, 150183, Institute of Microbiology and Infection, Birmingham, UK., Lawley TD; Host-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Forslund SK; Max Delbrück Center for Molecular Medicine (MDC), 13125, Berlin, Germany. sofia.forslund@mdc-berlin.de.; Experimental and Clinical Research Center, A Cooperation of Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Lindenberger Weg 80, 13125, Berlin, Germany. sofia.forslund@mdc-berlin.de.; DZHK (German Centre for Cardiovascular Research), Partner Site, 10785, Berlin, Germany. sofia.forslund@mdc-berlin.de.; Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Berlin, Germany. sofia.forslund@mdc-berlin.de.; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Structural and Computational Biology Unit, 69117, Heidelberg, Germany. sofia.forslund@mdc-berlin.de., Moffatt MF; National Heart and Lung Institute, Imperial College London, London, UK. m.moffatt@imperial.ac.uk., Cookson WOC; National Heart and Lung Institute, Imperial College London, London, UK. w.cookson@imperial.ac.uk.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Feb 12; Vol. 7 (1), pp. 171. Date of Electronic Publication: 2024 Feb 12.
DOI: 10.1038/s42003-024-05840-3
Abstrakt: Microbial communities at the airway mucosal barrier are conserved and highly ordered, in likelihood reflecting co-evolution with human host factors. Freed of selection to digest nutrients, the airway microbiome underpins cognate management of mucosal immunity and pathogen resistance. We show here the initial results of systematic culture and whole-genome sequencing of the thoracic airway bacteria, identifying 52 novel species amongst 126 organisms that constitute 75% of commensals typically present in heathy individuals. Clinically relevant genes encode antimicrobial synthesis, adhesion and biofilm formation, immune modulation, iron utilisation, nitrous oxide (NO) metabolism and sphingolipid signalling. Using whole-genome content we identify dysbiotic features that may influence asthma and chronic obstructive pulmonary disease. We match isolate gene content to transcripts and metabolites expressed late in airway epithelial differentiation, identifying pathways to sustain host interactions with microbiota. Our results provide a systematic basis for decrypting interactions between commensals, pathogens, and mucosa in lung diseases of global significance.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: