Automated differentiation of mixed populations of free-flying female mosquitoes under semi-field conditions.

Autor: Johnson BJ; Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. brian.johnson@qimrberghofer.edu.au., Weber M; Biogents AG, Weissenburgstr. 22, 93055, Regensburg, Germany., Al-Amin HM; Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia., Geier M; Biogents AG, Weissenburgstr. 22, 93055, Regensburg, Germany., Devine GJ; Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 12; Vol. 14 (1), pp. 3494. Date of Electronic Publication: 2024 Feb 12.
DOI: 10.1038/s41598-024-54233-3
Abstrakt: Great advances in automated identification systems, or 'smart traps', that differentiate insect species have been made in recent years, yet demonstrations of field-ready devices under free-flight conditions remain rare. Here, we describe the results of mixed-species identification of female mosquitoes using an advanced optoacoustic smart trap design under free-flying conditions. Point-of-capture classification was assessed using mixed populations of congeneric (Aedes albopictus and Aedes aegypti) and non-congeneric (Ae. aegypti and Anopheles stephensi) container-inhabiting species of medical importance. Culex quinquefasciatus, also common in container habitats, was included as a third species in all assessments. At the aggregate level, mixed collections of non-congeneric species (Ae. aegypti, Cx. quinquefasciatus, and An. stephensi) could be classified at accuracies exceeding 90% (% error = 3.7-7.1%). Conversely, error rates increased when analysing individual replicates (mean % error = 48.6; 95% CI 8.1-68.6) representative of daily trap captures and at the aggregate level when Ae. albopictus was released in the presence of Ae. aegypti and Cx. quinquefasciatus (% error = 7.8-31.2%). These findings highlight the many challenges yet to be overcome but also the potential operational utility of optoacoustic surveillance in low diversity settings typical of urban environments.
(© 2024. The Author(s).)
Databáze: MEDLINE
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