Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants.

Autor: Tausta SL; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06510, USA.; Institute of Biomolecular Design and Discovery, Yale University West Campus, West Haven, CT, 06516, USA., Fontaine K; Department of Radiology and Biomedical Imaging, Yale School of Medicine, 330 Cedar Street, New Haven, CT, 06519, USA.; Yale PET Center, Yale University, 801 Howard Avenue, New Haven, CT, 06510, USA., Hillmer AT; Department of Radiology and Biomedical Imaging, Yale School of Medicine, 330 Cedar Street, New Haven, CT, 06519, USA.; Yale PET Center, Yale University, 801 Howard Avenue, New Haven, CT, 06510, USA.; Department of Biomedical Engineering, Yale University, 17 Hillhouse Avenue, New Haven, CT, 06511, USA.; Department of Psychiatry, Yale School of Medicine, 300 George Street, New Haven, CT, 06510, USA., Strobel SA; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06510, USA. scott.strobel@yale.edu.; Institute of Biomolecular Design and Discovery, Yale University West Campus, West Haven, CT, 06516, USA. scott.strobel@yale.edu.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2024 Feb 11; Vol. 114 (1), pp. 17. Date of Electronic Publication: 2024 Feb 11.
DOI: 10.1007/s11103-023-01413-w
Abstrakt: Fluoride is an environmental toxin prevalent in water, soil, and air. A fluoride transporter called Fluoride EXporter (FEX) has been discovered across all domains of life, including bacteria, single cell eukaryotes, and all plants, that is required for fluoride tolerance. How FEX functions to protect multicellular plants is unknown. In order to distinguish between different models, the dynamic movement of fluoride in wildtype (WT) and fex mutant plants was monitored using [ 18 F]fluoride with positron emission tomography. Significant differences were observed in the washout behavior following initial fluoride uptake between plants with and without a functioning FEX. [ 18 F]Fluoride traveled quickly up the floral stem and into terminal tissues in WT plants. In contrast, the fluoride did not move out of the lower regions of the stem in mutant plants resulting in clearance rates near zero. The roots were not the primary locus of FEX action, nor did FEX direct fluoride to a specific tissue. Fluoride efflux by WT plants was saturated at high fluoride concentrations resulting in a pattern like the fex mutant. The kinetics of fluoride movement suggested that FEX mediates a fluoride transport mechanism throughout the plant where each individual cell benefits from FEX expression.
(© 2024. The Author(s).)
Databáze: MEDLINE
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