Effectiveness of photo-ozone therapy against equine Pythium insidiosum.

Autor: Rodrigues VS; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Trevisan LAC; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Cintra BS; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Pires RH; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Ribeiro AB; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Tavares DC; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600., Oberhaus E; School of Animal Sciences, Louisiana State University, 201D Animal & Food Sciences Lab Bldg., Baton Rouge, LA, USA, 70803., Ferreira JC; Post-graduation Program in Animal Sciences, University of Franca, 201 Dr. Armando de Sáles Oliveira, Franca, SP, Brazil, 14404-600. Electronic address: jair.ferreira@unifran.edu.br.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2024 Mar; Vol. 134, pp. 105030. Date of Electronic Publication: 2024 Feb 09.
DOI: 10.1016/j.jevs.2024.105030
Abstrakt: Cutaneous pythiosis is a life-threatening infectious disease. Low-level laser therapy (LLLT) and ozone (O 3 ) have been used individually in the treatment of infected wounds. The goals of the study were a) to characterize the antimicrobial action of the photo-ozone therapy (LLLT-O 3 ) against equine Pythium insidiosum, and b) to assess the cytotoxic potential of the LLLT-O 3 in keratinocytes. Specimens of pathogen were isolated from 10 horses. After culturing, 120 hyphae plugs were distributed among four groups (n=30 hyphae plugs/group): LLLT (laser irradiation for 160 sec;), O 3 (exposition to O 3 for 15 min;), LLLT-O 3 (LLLT and O 3 treatments in sequence) and control (untreated plugs). The hyphae growth was measured during the first 14 days post-treatment. Where there was an absence of hyphae growth, the plug was recultured for an additional 7 days. The cytotoxic potential of the treatments against HaCaT keratinocytes was assessed by colorimetric assays. The LLLT-O 3 and O 3 treatments inactivated, respectively, 92.3% (28/30) and 30% (9/30) of the samples. No growth was detected after 7 days reculture of inactivated hyphae plugs on new media. Hyphae growth was visualized in 100% of the control and LLLT hyphae plugs. The viability of HaCaT cells was not affected by the isolated treatments (LLLT and O 3 ), while the LLLT-O 3 showed slight cytotoxic effect (20%) when compared to the control group (P<0.05). Photo-ozone therapy inactivated equine P. insidiosum hyphae with minimal cytotoxicity in skin cells in vitro.
Competing Interests: Declaration of competing interest None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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