Immunomodulatory effect of Lacticaseibacillus casei CB054 supplementation in calves vaccinated against infectious bovine rhinotracheitis.

Autor: Brasil CL; Instituto de Biologia, Programa de Pós-Graduação em Microbiologia e Parasitologia, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. Electronic address: carolinalitchinabrasil@hotmail.com., Gonçalves VS; Biotecnologia - Centro de Desenvolvimento Tecnológico - Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. Electronic address: vitoriasgon@gmail.com., Santos FDS; Biotecnologia - Centro de Desenvolvimento Tecnológico - Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. Electronic address: denis.santos195@gmail.com., Rodrigues PRC; Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS 96010-900, Brazil. Electronic address: priccenteno@hotmail.com., Leite FPL; Instituto de Biologia, Programa de Pós-Graduação em Microbiologia e Parasitologia, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Biotecnologia - Centro de Desenvolvimento Tecnológico - Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. Electronic address: fleivasleite@gmail.com., Pereira DIB; Instituto de Biologia, Programa de Pós-Graduação em Microbiologia e Parasitologia, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. Electronic address: danielabrayer@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2024 Mar; Vol. 269, pp. 110726. Date of Electronic Publication: 2024 Feb 02.
DOI: 10.1016/j.vetimm.2024.110726
Abstrakt: Probiotics are live microorganisms that, confer health benefits to the host when supplemented in adequate amounts. They can promote immunomodulation by inducing phagocyte activity, leukocyte proliferation, antibody production, and cytokine expression. Lactic acid bacteria (BAL) are important probiotic specimens with properties that can improves ruminant nutrition, productivity and immunity. The aim of the present study was to evaluate the immunomodulatory effect of the supplementation with Lacticaseibacillus casei CB054 in calve vaccinated against bovine infectious rhinotracheitis (IBR). Calve were vaccinated with a commercial IBR vaccine, on day 0 and received a booster dose on day 21. L. casei CB054 was orally administered (4 ×10 9 UFC) for 35 days, while a non-supplemented control group received Phosphate Buffer Saline (PBS). Stimulation of bovine splenocytes with L. casei CB054 markedly enhanced mRNA transcription levels of cytokines IL2, IL4, IL10 and IL17 genes. Calves supplemented with L. casei CB054 showed significantly higher (p < 0.05) specific anti-BoHV-1 IgG levels, higher serum neutralization, as well as higher mRNA transcription for IL2, IL4, IL10 and IL17 genes in Peripheral Blood Mononuclear Cells (PBMCs) comparing with control calves. Supplemented calve had an average weight gain of ∼14 kg more than non-supplemented during the experimental period. These results suggest that L. casei CB054 supplementation increase immunogenicity of a commercial IBR vaccine in cattle and improve weight gain.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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