Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production.

Autor: Wisnu Prajoko Y; Department of Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia., Putra A; Stem Cell and Cancer Research, Semarang, Indonesia.; Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.; Department of Pathology Anatomy, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia., Prasetio A; Stem Cell and Cancer Research, Semarang, Indonesia., Dina Amalina N; Stem Cell and Cancer Research, Semarang, Indonesia.; Pharmaceutical Sciences Department, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia.
Jazyk: angličtina
Zdroj: Medicinski glasnik : official publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina [Med Glas (Zenica)] 2024 Feb 01; Vol. 21 (1), pp. 1-7. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.17392/1608-23
Abstrakt: Aim To determine the eff ect of hypoxic mesenchymal stem cells (MSCs) on the interleukin (IL)-10 and interferon (IFN)-gamma in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients. Methods This study used a post-test control group design. Hypoxic MSCs were isolated and characterized according to their surface marker expression and diff erentiation capacities. PBMCs isolated from SLE patients were divided into three groups: control and two treatment groups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10 (T1) and 1:1 (T2) for 48 h incubation. Furthermore, IFN-gamma and IL-10 levels were determined by cytometric bead array (CBA) fl ow cytometry. Results Hypoxic MSCs signifi cantly decreased the IFN-gamma levels and increased the IL-10 levels in dose-dependent manner compared to the control group. The highest activity of hypoxic MSCs was noticed in T2 group. Conclusion Hypoxic MSCs- induced IL-10 are important in the control of anti-infl ammatory eff ect on SLE through inhibiting IFN-gamma.
(Copyright© by the Medical Assotiation of Zenica-Doboj Canton.)
Databáze: MEDLINE
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