Soil exposure modulates the immune response to an influenza challenge in a mouse model.

Autor: McCumber AW; Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, Chapel Hill, NC, USA., Kim YJ; Civil and Environmental Engineering Department, Duke University, Durham, NC, USA., Granek J; Department of Biostatistics and Bioinformatics, Duke University, Durham, NC, USA., Tighe RM; Department of Medicine, Duke University, Durham, NC, USA., Gunsch CK; Civil and Environmental Engineering Department, Duke University, Durham, NC, USA. Electronic address: ckgunshc@duke.edu.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Apr 20; Vol. 922, pp. 170865. Date of Electronic Publication: 2024 Feb 08.
DOI: 10.1016/j.scitotenv.2024.170865
Abstrakt: There is increasing evidence that early life microbial exposure aids in immune system maturation, more recently known as the "old friends" hypothesis. To test this hypothesis, 4-week-old mice were exposed to soils of increasing microbial diversity for four weeks followed by an intranasal challenge with either live or heat inactivated influenza A virus and monitored for 7 additional days. Perturbations of the gut and lung microbiomes were explored through 16S rRNA amplicon sequencing. RNA-sequencing was used to examine the host response in the lung tissue through differential gene expression. We determined that compared to the gut microbiome, the lung microbiome is more susceptible to changes in beta diversity following soil exposure with Lachnospiraceae ASVs accounting for most of the differences between groups. While several immune system genes were found to be significantly differentially expressed in lung tissue due to soil exposures, there were no differences in viral load or weight loss. This study shows that exposure to diverse microbial communities through soil exposure alters the gut and lung microbiomes resulting in differential expression of specific immune system related genes within the lung following an influenza challenge.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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