Autor: |
Paschalidis K; Department of Agriculture, School of Agricultural Sciences, Hellenic Mediterranean University, 71410 Heraklion, Greece., Fanourakis D; Department of Agriculture, School of Agricultural Sciences, Hellenic Mediterranean University, 71410 Heraklion, Greece., Tsaniklidis G; Hellenic Agricultural Organization (ELGO-DIMITRA), Institute of Olive Tree, Subtropical Crops and Viticulture, 73134 Chania, Greece., Tsichlas I; Department of Agriculture, School of Agricultural Sciences, Hellenic Mediterranean University, 71410 Heraklion, Greece., Tzanakakis VA; Department of Agriculture, School of Agricultural Sciences, Hellenic Mediterranean University, 71410 Heraklion, Greece., Bilias F; Soil Science Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece., Samara E; Soil Science Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece., Ipsilantis I; Soil Science Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece., Grigoriadou K; Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, 57001 Thessaloniki, Greece., Samartza I; Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, 57001 Thessaloniki, Greece., Matsi T; Soil Science Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece., Tsoktouridis G; Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, 57001 Thessaloniki, Greece.; Theofrastos Fertilizers, Industrial Area of Korinthos, Irinis & Filias, Ikismos Arion, Examilia, 20100 Korinthos, Greece., Krigas N; Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, 57001 Thessaloniki, Greece. |
Abstrakt: |
Herein, we applied DNA barcoding for the genetic characterization of Sideritis syriaca subsp. syriaca (Lamiaceae; threatened local Cretan endemic plant) using seven molecular markers of cpDNA. Five fertilization schemes were evaluated comparatively in a pilot cultivation in Crete. Conventional inorganic fertilizers (ChFs), integrated nutrient management (INM) fertilizers, and two biostimulants were utilized (foliar and soil application). Plant growth, leaf chlorophyll fluorescence, and color were assessed and leaf content of chlorophyll, key antioxidants (carotenoids, flavonoids, phenols), and nutrients were evaluated. Fertilization schemes induced distinct differences in leaf shape, altering quality characteristics. INM-foliar and ChF-soil application promoted yield, without affecting tissue water content or biomass partitioning to inflorescences. ChF-foliar application was the most stimulatory treatment when the primary target was enhanced antioxidant contents while INM-biostimulant was the least effective one. However, when the primary target is yield, INM, especially by foliar application, and ChF, by soil application, ought to be employed. New DNA sequence datasets for the plastid regions of pet B/ pet D, rpo C1, psb K- psb I, and atp F/ atp H were deposited in the GenBank for S. syriaca subsp. syriaca while the molecular markers rbc L, trn L/ trn F, and psb A/ trn H were compared to those of another 15 Sideritis species retrieved from the GenBank, constructing a phylogenetic tree to show their genetic relatedness. |